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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (4): 274-277.doi: 10.3736/jcim20040412

• Original Experimental Research • Previous Articles     Next Articles

Expression of TIMP-1 and TGF-β1 mRNA in hepatic fibrosis rats and the therapeutic effects of Bushen Rougan Recipe

Bin Zhang1(), Mo-bin Wan1, Lin-tai Wang2   

  1. 1. Department of Infectious Diseases,Changhai Hospital, Second Military Medical University,Shanghai 200433, China
    2. Department of Liver Diseases, Shuguang Hospital,Shanghai University of Traditional Chinese Medicine, Shanghai 200021,China
  • Received:2004-04-19 Online:2004-07-20 Published:2018-10-20

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Objective: To study the expression of TIMP-1 and TGF-β1 mRNA in hepatic fibrosis rats and the therapeutic effects of Bushen Rougan Recipe (BSRGR).Methods: Hepatic fibrosis was induced in rats by dimethylnitrosamine (DMN). The rats' hepatic tissue was studied by HE staining and Sirius red staining. The rats were divided into normal control group, fibrosis model group and BSRGR treated group (10 g·kg -1·d -1, i.g. for 4 weeks). At last, TIMP-1 and TGF-β1 mRNA were detected by RT-PCR. Results: Pathological study showed that hepatic fibrosis was successfully induced by DMN in rats. The expression quantity of TIMP-1 and TGF-β1 mRNA were the most in the fibrosis model group, the second in the BSRGR treated group,and the least in the normal control group.Conclusion: The expression of TIMP-1 and TGF-β1mRNA was increased in the hepatic fibrosis rats, and this is one possible mechanism of hepatic fibrosis. BSRGR can inhibit the advancement of hepatic fibrosis.

Key words: hepatic fibrosis, Bushen Rougan Recipe, dimethylnitrosamine, TIMP-1, TGF-β1

CLC Number: 

  • R285.5

Tab 1

Prime sequences for PCR"

Prime Sn An Length （bp）
TIMP-1 CCACAGATATCCGGTTCGCCTACA GCACACCCCACAGCCAGCACTAT 218
TGF-β1 AGGCGGTGCTCGCTTTGT CCCGAATGTCTGACGTATTGAA 201
β-actin TCACCCACACTGTGCCCATCTACGA TCACCCACACTGTGCCCATCTACGA 300

Fig 1

Sirius red staining of liver tissue（×100）"

Tab 2

The degree of liver fibrosis"

Group n Degree of liver fibrosis Ridit analysis
– + ++ +++ ++++
Normal control 10 10 P<0.01
Fibrosis model 9 0 0 4 2 3
BSRGR-treated 12 0 0 6 5 1 P<0.05

Tab 3

Expression level of TIMP-1 and TGF-β1 mRNA ($\bar{x}$±s )"

Group n TIMP-1/β-actin TGF-β1/β-actin
Normal control 3 0.523±0.30** 0.663±0.10**
Fibrosis model 3 0.823±0.45△△ 1.100±0.21△△
BSRGR-treated 3 0.617±0.02**△△ 0.812±0.04**

Fig 2

Agrose gel electrophoresis of TIMP-1 PCR products Lane 1: Marker(3 000, 2 000, 1 500, 1 200, 1 031, 900, 800, 700, 600, 500, 400, 300, 200, 100 bp); Lane 2-4: Amplified products of β-actin (300 bp); Lane 5-7: Amplified products of normal control group, fibro-sis model group and BSRGR treated group, respectively (218 bp)."

Fig 3

Agrose gel electrophoresis of TGF-β1 PCR products Lane 1: Marker (3 000, 2 000, 1 500, 1 200, 1 031, 900, 800, 700, 600, 500, 400, 300, 200, 100 bp); Lane 2-4: Amplified products of β-actin (300 bp); Lane 5-7: Amplified products of normal control group, fibro-sis model group and BSRGR treated group, respectively (201 bp)."

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