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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (3): 285-288.doi: 10.3736/jcim20060313

• Original Experimental Research • Previous Articles     Next Articles

Inhibiting effects of root of Mallotus apelta on duck hepatitis B virus

Shu XU, Zhi-ping Lv, Hong-bing Cai, Xiao-gang Zhang, Qiang Liu, Yan Tan   

  1. School of Traditional Chinese Medicine, Nanfang Medical University, Guangzhou, Guangdong Province 510515, China
  • Online:2006-05-31 Published:2006-05-15

Objective

To evaluate the inhibiting effects of the root of Mallotus apelta (Lour.) Muell.-Arg. on duck hepatitis B virus (D-HBV) in vivo.

Methods

Forty nestling ducks with congenitally infection of D-HBV detected by PCR were randomly divided into five groups: untreated group, lamivudine-treated group, and high-, medium- and low-dose root of Mallotus apelta-treated groups. The ducks in the lamivudine-treated group were fed lamivudine with a dose of 50 mg/kg once. Ducks in the three-dose Mallotus apelta-treated groups were treated with different doses of decoction of this herbal medicine for 21 days respectively. The serum content of D-HBV DNA was determined by quantitative real-time PCR technique before and 7 days after the treatment, and on the 7th, 14th and 21st day of the treatment. Liver biopsy was also executed before and after the treatment to observe the histopathological changes.

Results

Lamivudine showed a rapid inhibiting effect on D-HBV DNA, but this effect didn't last long, and the serum level of D-HBV DNA increased again after treatment. The serum level of D-HBV DNA dropped markedly in the high- and medium-dose Mallotus apelta-treated groups on the 14th and 21st day. Low-dose Mallotus apelta revealed no obvious inhibiting effect on D-HBV. After treatment, the inhibiting effect in the root of Mallotus apelta-treated group continued as compared with that in the untreated group. The histopathological changes of liver tissues showed that the inflammation in the high-dose root of Mallotus apelta-treated group was weakened as compared with that in the lamivudine-treated group.

Conclusion

The root of Mallotus apelta has therapeutic effect on D-HBV. It can restrain the duplication of D-HBV in vivo. Although this effect is weaker than that of lamivudine, it continues longer. Thus this herbal medicine is an effective, safe and economical drug for hepatitis B.

Key words: Drugs, Chinese herbal, Root of Mallotus apelta (Lour.) Muell.-Arg., Duck hepatitis B virus, Quantitative real-time PCR, Animal experimentation

CLC Number: 

  • R512.62

Figure 1

Electrophoregram of PCR products of D-HBVA & B: Positive samples; C & D: Positive-detected samples."

Table 1

Copy number of D-HBV DNA in serum of nestling ducks before, during and after treatment in different groups(ヌ±S, copy/ml)"

Group n Copy number of D-HBV DNA
Pre-treatment Treatment for 7 days Treatment for 14 days Treatment for 21 days 7 days after treatment
Untreated 8 (1.95±0.95)×108 (1.73±0.74)×108 (1.47±0.71)×108 (6.64±0.77)×108 (1.45±0.75)×108*
High-dose M. 8 (1.92±1.13)×108 (1.80±1.09)×108▲▲ (6.69±2.84)×107* (4.97±4.83)×107*△△ (4.02±3.53)×107*
apelta-treated
Medium-dose M. 8 (1.87±0.90)×108 (1.54±0.69)×108▲▲ (7.48±4.81)×107△▲▲ (5.66±4.14)×107*△△ (4.87±4.27)×107*
apelta-treated
Low-dose M. 8 (1.73±0.66)×108 (1.52±0.61)×108▲▲ (1.51±0.61)×108▲▲ (1.36±0.47)×108▲▲ (1.28±0.65)×108*
apelta-treated
Lamivudine- 8 (1.68±1.17)×108 (1.14±0.57)×107*△△ (6.35±8.79)×106*△△ (3.77±3.43)×106*△△ (6.82±6.39)×107
treated

Figure 2

Histopathological changes of liver tissue in nestling ducks with D-HBV infection before and after treatment (HE staining, ×400)A: Pre-treatment; B: Lamivudine-treated group after treatment; C: High-dose Mallotus apelta-treated group after treatment."

Figure 3

Histopathological changes of liver tissue in nestling ducks with D-HBV infection beforeand after treatment (aceto-orcein staining, ×400)A: Pre-treatment; B: Lamivudine-treated group after treatment; C: High-dose Mallotus apelta-treated group after treatment."

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