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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (3): 255-260.doi: 10.3736/jcim20090311

• Original Experimental Research • Previous Articles     Next Articles

Inhibitory effects of Feiyanning Decoction on proliferation, migration and tube formation of human umbilical vein endothelial cells in vitro

 Hai-bin Deng, Zhong-qi Wang, Zhen-ye Xu   

  1. No. 2 Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2008-09-01 Accepted:2008-12-08 Online:2009-03-20 Published:2009-03-15

Objective

To observe the inhibitory effects of Feiyanning Decoction, a compound traditional Chinese herbal medicine for replenishing qi, nourishing essence, and diminishing stagnation by detoxification, on proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVECs).
Methods

HUVECs were isolated from the new-born umbilical core of human. Serum containing Feiyanning was prepared in SD rats by oral gavage of the Feiyanning Decoction for three days. The inhibitory effects of different contents of serum containing Feiyanning on the proliferation of HUVECs, human lung adenocarcinoma cell A549, and HUVECs cultured with A549-conditioned media were observed by sulforhodamine B (SRB) method; the effects on migration of HUVECs were inspected by using Boyden Chamber Transwell method, and the effects on tube formation of HUVECs were evaluated by cavity forming experiment.
Results

Serum containing Feiyanning at a concentration of 25%, 37.5% or 50% inhibited the proliferation of both the HUVECs and the HUVECs cultured with A549-conditioned media (P<0.01, P<0.05). High concentration serum containing Feiyanning inhibited the proliferation of A549 cells. Sera containing Feiyanning at concentrations of 12.5% to 37.5% inhibited the migration of HUVECs induced by 20% fetal bovine serum (P<0.05, P<0.01) and the formation of the primary tube of HUVECs.
Conclusion

Feiyanning Decoction can inhibit the angiogenesis of HUVECs, which may be one of its mechanisms in inhibiting the invasion metastases of lung cancer.

Key words: Feiyanning Decoction, Human umbilical vein endothelial cell, Cell proliferation, Cell movement, Neovascularization, Pathologic

Table 1

Effects of serum containing Feiyanning on proliferation of HUVECs and A549 cells after 48-hour treatment (x±s)"

Group n HUVECs A549 cells
OD (×10–2) Suppression ratio (%) OD (×10–2) Suppression ratio (%)
Control 9 75.98±5.58 0 99.41±5.03 0
Control serum
12.5% 9 64.98±6.70* 14.56±7.96 75.00±3.94** 24.55±1.97
25% 9 64.09±7.65* 15.74±9.04 87.58±2.99** 11.90±2.32
37.5% 9 61.59±6.06** 19.00±6.56 85.77±2.89** 13.72±0.76
50% 9 55.72±2.67** 25.33±2.77 86.00±3.32** 13.49±0.90
Serum containing Feiyanning
12.5% 9 45.36±4.93**△△ 40.29±2.26 74.51±3.52** 25.05±0.70
25% 9 27.53±2.81**△△ 63.76±1.29 72.54±3.81**△△ 27.04±1.61△△
37.5% 9 21.77±7.05**△△ 71.32±3.00△△ 55.86±15.49**△△ 43.80±2.97△△
50% 9 11.60±1.26**△△ 84.73±0.70△△ 46.34±2.99**△△ 53.37±1.35△△

Figure 1

Effects of A549-conditioned media on HUVECs cultured for different time"

Table 2

Effects of serum containing Feiyanning on proliferation of HUVECs in A549-conditioned media (x±s)"

Group n OD Suppression
ratio (%)
Control 9 1.07±0.20 0
Control serum
12.5% 9 1.04±0.18 2.70±2.15
25% 9 0.99±0.13 6.59±6.29
37.5% 9 1.01±0.19 5.73±1.44
50% 9 1.02±0.20 4.67±2.23
Serum containing Feiyanning
12.5% 9 0.65±0.11**△△ 39.65±1.33△△
25% 9 0.52±0.10**△△ 51.59±2.66
37.5% 9 0.40±0.12**△△ 63.38±3.13△△
50% 9 0.31±0.09**△△ 71.69±2.21△△

Table 3

Effects of serum containing Feiyanning on migration of HUVECs (x±s)"

Group n OD Suppression ratio (%)
Control 9 1.27±0.22 0
Control serum
12.5% 9 1.24±0.17 2.24±4.19
25% 9 1.22±0.19 4.22±2.27
37.5% 9 1.14±0.20 10.39±0.82
Serum containing Feiyanning
12.5% 9 0.84±0.24**△△ 35.18±7.41
25% 9 0.76±0.22**△△ 41.43±6.54
37.5% 9 0.58±0.13**△△ 54.66±2.35△△

Figure 2

Serum containing Feiyanning inhibits formation of primary tube of HUVECs on matrigel in vitro (Inverted microscopy, ×200) A: Control group; B: 12.5% serum containing Feiyanning group; C: 25% serum containing Feiyanning group; D: 37.5% serum containing Feiyanning group."

[1] Xu ZY, Yang YF. Lung cancer: combined therapy with traditional Chinese and Western medicine[M]. Beijing: People’s Medical Publishing House,2002: 8-9
徐振晔, 杨宇飞 . 肺癌中西医综合治疗[M]. 北京: 人民卫生出版社,2002: 8-9
[2] Wang ZQ, Xu ZY, Zhou WD, Deng HB, Zhang M, Zhang H . A clinic study on non-small cell lung cancer standard treatment with integrated traditional Chinese and Western medicine[J]. Zhong Yi Yao Tong Bao, 2006,5(3):39-43
王中奇, 徐振晔, 周卫东, 邓海滨, 张铭, 张晖 . 西医结合规范化治疗非小细胞肺癌的临床研究[J]. 中医药通报, 2006,5(3):39-43
[3] Wang ZQ, Xu ZY, Deng HB, Zhang M . Experimental study on “Feiyanning Decoction” in preventing angiogenesis of Lewis tumor in C57 mice[J]. Shanghai Zhong Yi Yao Za Zhi, 2006,40(7):66-67
王中奇, 徐振晔, 邓海滨, 张铭 . 肺岩宁方抗C57小鼠Lewis肿瘤血管生成的实验研究[J]. 上海中医药杂志, 2006,40(7):66-67
[4] Jaffe EA, Nachman RL, Becker CG, Minick CR . Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria[J]. J Clin Invest, 1973,52(11):2745-2756
doi: 10.1172/JCI107470
[5] Lei Y, Chen KJ, Li ZW, Xu YG, Liu JG . Study on relationship of clinical effect of Xuefu Zhuyu concen-trated pill and serum pharmacological anti-platelet activating effect in vitro[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2002,22(4):270-273
雷燕, 陈可冀, 李中文, 许勇钢, 刘剑刚 . 血府逐瘀浓缩丸抗血小板活化的临床疗效与体外血清药理作用的相关性研究[J]. 中国中西医结合杂志, 2002,22(4):270-273
[6] Wang LQ, Li YK, Fu SG, Fu HD . A study on serum pharmacology research methods[J]. Zhong Yao Yao Li Yu Lin Chuang, 1997,13(3):29-31
王力倩, 李仪奎, 符胜光, 付惠娣 . 血清药理学方法研究探索[J]. 中药药理与临床, 1997,13(3):29-31
[7] Chen Q. Technology of research on pharmacology of traditional Chinese medicine[M]. Beijing: People’s Medical Publishing House,1994: 33-34
陈奇 . 中药药理研究方法学[M]. 北京: 人民卫生出版社,1994: 33-34
[8] Wang JJ, Wang B, Guo J, Xu Q, Gao Y, Zhu CL . Inhibitory effect of ginsenoside Rg3 on proliferation of endothelial cells induced by gastric cancer[J]. Lin Chuang Zhong Liu Xue Za Zhi, 1999,4(2):68
王杰军, 王兵, 郭静, 许青, 高勇, 朱长力 . 人参皂甙Rg3对胃癌诱导血管内皮细胞增殖的抑制作用[J]. 临床肿瘤学杂志, 1999,4(2):68
[9] Koyama S, Takagi H, Otani A, Suzuma K, Nishimura K, Honda Y . Tranilast inhibits protein kinase C-dependent signalling pathway linked to angiogenic activities and gene expression of retinal microcapillary endothelial cells[J]. Br J Pharmacol, 1999,127(2):537-545
doi: 10.1038/sj.bjp.0702564
[10] Ashton AW, Yokota R, John G, Zhao S, Suadicani SO, Spray DC, Ware JA . Inhibition of endothelial cell migration, intercellular communication, and vascular tube formation by thromboxane A2[J]. J Biol Chem, 1999,274(50):35562-35570
doi: 10.1074/jbc.274.50.35562
[11] Shibuya M . Differential roles of vascular endothelial growth factor receptor-1 and receptor-2 in angiogenesis[J]. J Biochem Mol Biol, 2006,39(5):469-478
[12] Keller G, Lacaud G, Robertson S . Development of the hematopoietic system in the mouse[J]. Exp Hematol, 1999,27(5):777-787
doi: 10.1016/S0301-472X(99)00024-7
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