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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (8): 817-820.doi: 10.3736/jcim20080809

• Original Experimental Research • Previous Articles     Next Articles

Effects of Radix notoginseng extracts drug-containing serum on expressions of bcl-2, Bax and p21WAF1 proteins in MNNG transformed GES-1 cells

Jun-xiang Li1(), Zhi-bin Wang1, Ling-qun Zhu2, Fu-ling Niu2, Wei Cui2   

  1. 1. Department of Gastroenterology, Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China
    2. Key Laboratory of Internal Medicine, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
  • Received:2007-12-10 Online:2008-08-20 Published:2008-08-15

Objective: To investigate the effects of Radix notoginseng extracts drug-containing serum on the expressions of apoptosis-regulating proteins including Bax, bcl-2 and p21WAF1 in precancerous gastric cells.
Methods: The N-methyl-N'-nitro-N-nitroso-guanidine (MNNG) transformed eternalized human gastric mucosa epithelium GES-1 cell line (MC cell) was used in vitro as a model of gastric precancerous lesion. The medicated canine serum was prepared by feeding to the adult Beagle dog with Radix notoginseng extracts and obtaining the serum after 2-hour medication. MC cells were cultured with medicated canine serum (medicated serum group) or non-medicated canine serum (normal control group) for 72 hours. Expressions of Bax, bcl-2 and p21 WAF1 proteins were detected by immunocytochemical assay and the average optical density of the cells was determined by an image analysis system.
Results: Compared with those of the normal control group, Bax and p21 WAF1 expressions in medicated serum group were significantly enhanced (P<0.01), while the expression of bcl-2 was significantly reduced (P<0.01).
Conclusion: Radix notoginseng extracts may inhibit the proliferation and promote the apoptosis of precancerous gastric cells through altering expressions of the bcl-2, Bax and p21 WAF1 genes.

Key words: Radix notoginseng, gastric cancer, bcl-2, Bax, p21 WAF1

CLC Number: 

  • R285.5

Figure 1

Expression of bcl-2 protein in MC cells (Immunocytochemical staining, ×400) A: Normal control group; B: Medicated serum group."

Figure 2

Expression of Bax protein in MC cells (Immunocytochemical staining, ×400) A: Normal control group; B: Medicated serum group."

Figure 3

Expression of p21WAF1 protein in MC cells (Immunocytochemical staining, ×400) A: Normal control group; B: Medicated serum group."

"

Group n Optical density
bcl-2 Bax p21WAF1
Normal control 7 0.843±0.053 0.482±0.021 0.035±0.024
Medicated serum 7 0.325±0.031** 0.645±0.037** 0.246±0.028**
[1] Wang ZB, Li JX, Zhu LQ , et al. Inhibiting effects of Radix notoginseng extracts on proliferation of GES-1 cells and MNNG-transformed GES-1 cells[J]. Zhong Xi Yi Jie He Xue Bao, 2004,2(6):445-449
王志斌, 李军祥, 朱陵群 , 等. 三七提取物对GES-1细胞及MNNG转化后GES-1细胞增殖的抑制作用[J]. 中西医结合学报, 2004,2(6):445-449
[2] Li JX, Wang ZB, Zhu LQ , et al. Cell proliferation inhibitive and apoptosis promoting effects of sanchi extract on GES-1 cell after being transformed by MNNG[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2005,25(8):719-722
doi: 10.7661/CJIM.2005.8.719
李军祥, 王志斌, 朱陵群 , 等. 三七提取物对经MNNG转化后的GES-1细胞增殖抑制及促凋亡作用[J]. 中国中西医结合杂志, 2005,25(8):719-722
doi: 10.7661/CJIM.2005.8.719
[3] Shi XY, Zhao FZ, Dai X , et al. Morphologic observation of Sanqi on rat precancerous lesion with experimental chronic atrophic gastritis[J]. Beijing Zhong Yi Yao Da Xue Xue Bao, 1999,22(6):45-47
doi: 10.3321/j.issn:1006-2157.1999.06.017
石雪迎, 赵凤志, 戴欣 , 等. 三七对大鼠实验性慢性萎缩性胃炎癌前病变作用的形态学观察[J]. 北京中医药大学学报, 1999,22(6):45-47
doi: 10.3321/j.issn:1006-2157.1999.06.017
[4] Sundblad AS, Tamayo R . Expression of MIB-1/KI-67 and bcl-2 in gastric carcinoma. Relationship with clinico-pathological factors[J]. Acta Gastroenterol Latinoam, 1995,25(2):67-72
[5] Xu AG, Li SG, Liu JH , et al. Significance of apoptosis and expression of bcl-2 in gastric pre-carcinoma and cancer[J]. Guangdong Yi Xue, 1998,19(7):489-490
徐岸高, 李绍光, 刘集红 , 等. 细胞凋亡及Bcl-2蛋白表达在胃癌发生中的意义[J]. 广东医学, 1998,19(7):489-490
[6] Yin C, Knudson CM, Korsmeyer SJ , et al. Bax suppression tumorigenesis and stimulates apoptosis in vivo[J]. Nature, 1997,385(6617):637-640
doi: 10.1038/385637a0
[7] el-Deiry WS, Tokino T, Velculescu VE , et al. WAF1, a potential mediator of p53 tumor suppression[J]. Cell, 1993,75(4):817-825
doi: 10.1016/0092-8674(93)90500-P pmid: 8242752
[8] Jiang H, Lin J, Su ZZ , et al. The melanoma differentiation-associated gene mda-6, which encodes the cyclin-dependent kinase inhibitor p21, is differentially expressed during growth, differentiation and progression in human melanoma cells[J]. Oncogene, 1995,10(9):1855-1864
[9] Kagawa S, Fujiwara T, Hizuta A , et al. p53 expression overcomes p21WAF1/CIP1-mediated G1 arrest and induces apoptosis in human cancer cells[J]. Oncogene, 1997,15(16):1903-1909
doi: 10.1038/sj.onc.1201362
[10] Frey RS, Li J, Singletary KW . Effects of genistein on cell proliferation and cell cycle arrest in nonneoplastic human mammary epithelial cells: involvement of Cdc2, p21(waf/cip1), p27(kip1), and Cdc25C expression[J]. Biochem Pharmacol, 2001,61(8):979-989
doi: 10.1016/S0006-2952(01)00572-X
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