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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (4): 392-396.doi: 10.3736/jcim20060415

• Original Experimental Research • Previous Articles     Next Articles

Radiosensitization of β-elemene on VX2 carcinoma transplanted on kidney in rabbits in vivo

Jun-jun She1,2, Zi-ming Wang1, Xiang-ming Che2, Cheng-en Pan2   

  1. 1. Department of Urology, Second Hospital, School of Medicine, Xi’an Jiaotong University,Xi’an, Shaanxi Province 710004, China;
    2. Department of General Surgery, First Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an, Shaanxi Province 710061, China;
  • Online:2006-07-31 Published:2006-07-15

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Objective

To investigate the radiosensitization of β-elemene on VX2 carcinoma transplanted on kidney in rabbits in vivo.

Methods

The rabbits were all transplanted with VX2 carcinoma on kidney. The appropriate dose of β-elemene infusion via renal artery for further study on radiosensitization was determined. Then fifty-five rabbits were divided into three groups: untreated group, radiation group and radiation plus β-elemene-treated group. After corresponding intervention for each group, the tumor volume was measured by ultrasonography and spiral computed tomography. The sensitization enhancement ratio (SER) of β-elemene was calculated. The pathological change of tumor tissue in kidey was observed by light microscopy and electron transmission microscopy. The apoptotic index was also examined by TdT-mediated dUTP-biotin nick end labeling method.

Results

The most significant radiosensitivity was observed in the radiation plus β-elemene-treated group with 6 Mev X-ray radiation dose of 3 Gy·Fx –1·d –1×5 d and β-elemene dose of 10 mg·kg –1·d –1. The average time delayed for tumor growth was obviously longer in the radiation plus β-elemene-treated group than those in the untreated group and radiation group. The SER of β-elemene was 1.89. The apoptotic index of tumor cells in the radiation plus β-elemene-treated group was also significantly higher than those in the untreated group and radiation group.

Conclusion

The β-elemene can enhance the effects of irradiation on VX2 carcinoma transplanted to kidney in rabbits in vivo by inducing apoptosis of tumor cells.

Key words: β-elemene, VX2 carcinoma transplanted in rabbits, Radiosensitization, Apoptosis

CLC Number: 

  • R737.11

Table 1

Effects of infusion of β-elemene with different doses via renal artery on growth of VX2 carcinoma transplanted in rabbits（ヌ±S, d）"

Group n Average time for enlargement of tumor Average time delayed
volume (from 3 cm3 to 10 cm3) for tumor growth
Untreated 5 18.8±1.00 –
β-elemene-treated
5 mg·kg–1·d–1 5 19.5±1.05 0.7±0.05
10 mg·kg–1·d–1 5 19.7±1.20 0.9±0.20
15 mg·kg–1·d–1 5 20.5±1.10* 1.7±0.10*
20 mg·kg–1·d–1 5 21.3±1.35** 2.5±0.35**

Table 2

Effects of radiation therapy (6 Mev X-ray) with different doses and infusion of β-elemene with different doses via renal artery on growth of VX2 carcinoma transplanted in rabbits（ヌ±S, d）"

Group n Average time for enlargement of tumor Average time delayed
volume (from 3 cm3 to 10 cm3) for tumor growth
Untreated 5 18.8±1.00 –
Radiation
1 Gy·Fx–1·d–1×5 d 5 19.2±1.00 0.4±0.00
2 Gy·Fx–1·d–1×5 d 5 20.0±1.10 1.2±0.10
3 Gy·Fx–1·d–1×5 d 5 20.9±1.30* 2.1±0.30*
4 Gy·Fx–1·d–1×5 d 5 21.4±1.89* 2.6±0.89*
5 Gy·Fx–1·d–1×5 d 5 23.6±1.78** 4.8±0.78**
Radiation plus β-elemene-treated (10 mg·kg–1·d–1)
1 Gy·Fx–1·d–1×5 d 5 19.4±1.00 0.6±0.00
2 Gy·Fx–1·d–1×5 d 5 20.7±1.15* 1.9±0.15*
3 Gy·Fx–1·d–1×5 d 5 23.0±2.13**△ 4.2±1.13**△
4 Gy·Fx–1·d–1×5 d 5 23.9±2.20**△ 5.1±1.20**△
5 Gy·Fx–1·d–1×5 d 5 24.5±2.01** 5.7±1.01**

Figure 1

Dose-effect curve of radiation therapy combined with β-elemene infusion via renal artery"

Figure 2

Ultrastructure of VX2 carcinoma transplanted in rabbits in different groups（×5 000）A: Untreated group; B: Radiation group; C: Radiation plus β-elemene-treated group."

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