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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (7): 738-743.doi: 10.3736/jcim20080715

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Bushen Jianpi Huoxue Recipe in prevention and treatment of tibial stress injuries in rabbits

You-xue Chen1(), Guo-ping Li2, Hong-en Yao1, Su-fan Li2, Li-gong Duan2   

  1. 1. Research Unit of Health Rehabilitation, Department of Basic Theory, Capital College of Physical Education, Beijing 100088,China
    2. Department of Traumatic Surgery, Beijing Sports Hospital, National Research Institute of Sports Medicine, Beijing 100061, China
  • Received:2007-09-05 Online:2008-07-20 Published:2008-07-15
  • Contact: CHEN You-xue

Objective: To explore the effects of Bushen Jianpi Huoxue Recipe, a compound traditional Chinese herbal medicine for tonifying the kidney, invigorating the spleen and promoting blood circulation, on tibial stress injuries in rabbits.
Methods: Thirty-five mature male rabbits were used in the experiment, and randomly divided into 7 groups: sedentary control (SC) group, 1-week exercise (E-1W) group, 2-week exercise (E-2W) group, 3-week exercise (E-3W) group, 4-week exercise (E-4W) group, 3-week exercise and 1-week Chinese herbal medicine treatment (EMT-1W) group, and 4-week exercise and 2-week Chinese herbal medicine treatment (EMT-2W) group. There were 5 rabbits in each group. A rabbit model of tibial stress injuries was established by stimulating the rabbits to jump and run within a high-voltage and low-current electronic cage. Radiologic features, bone scintigraphy, histology and electron microscopy of rabbit tibia were observed, and the content of blood parathyroid hormone (PTH), bone gla protein (BGP) and testosterone was detected by using radioimmunoassay method.
Results: After 2-week exercise, changes in histology and osteocytes had a tendency towards stress injuries. Serum PTH and BGP levels were remarkably increased, while serum testosterone level was lower than that in the SC group. Three- and four-week continuous exercise resulted in tibial stress injuries, and the positive changes were observed in X-ray features and radionuclide images. Compared with E-3W group, the levels of serum BGP and testosterone were decreased remarkably. A series of positive results such as prevailing negativeness of X-ray features and radionuclide images, increasing process of osteogenesis, typical osteogenic phase of osteocytes and favorable transformation of biochemical markers was shown in EMT-1W and EMT-2W groups. It also showed remarkable rising levels of serum BGP and testosterone and remarkable reducing level of serum PTH in EMT-1W and EMT-2W groups as compared with E-1W and E-2W groups.
Conclusion: Bushen Jianpi Huoxue Recipe can prevent and treat exercise-induced tibial stress injuries by stimulating new bone formation and increasing serum testosterone level.

Key words: tibial, kidney-reinforcing drugs, bone remodeling, cellular structure, biochemical markers, rabbits

CLC Number: 

  • R683

Table 1

Changes of bone scintigraphy in different groups"

Group n Grade of bone scintigraphy
SC 5 Negative
E-1W 5 Negative
E-2W 5 Negative
E-3W 5 Positive (stage Ⅰ) (two cases)
E-4W 5 Positive (stage Ⅰ, Ⅱ) (three cases)
EMT-1W 5 Positive (stage Ⅰ) (one case)
EMT-2W 5 Positive (stage Ⅰ) (one case)

Table 2

Changes of Howship's lacuna area in different groups ($\bar{x}$±s, μm2)"

Group n Area of Howship's lacunae
SC 5 7 693.06±1 658.95
E-1W 5 18 276.40±4 623.07*
E-2W 5 29 577.83±3 964.36**
E-3W 5 33 350.81±5 955.07**
E-4W 5 32 632.04±3 993.92**
EMT-1W 5 22 833.44±5 825.08
EMT-2W 5 19 359.76±2 974.52

Table 3

Changes of serum concentrations of parathyroid hormone, bone gla protein and testosterone in different groups ($\bar{x}$±s)"

Group n PTH (ng/L) BGP (μg/L) Testosterone (ng/L)
SC 5 28.4±10.2 137.74±34.04 1 692.3±132.5
E-1W 5 239.5±11.6** 172.18±11.84 1 492.6±296.5*
E-2W 5 168.3±41.8** 251.07±43.36* 1 128.1±176.7**
E-3W 5 125.5±12.7** 205.60±56.77 1 388.9±421.1*
E-4W 5 306.9±97.3** 159.81±33.26 1 323.2±192.3*
EMT-1W 5 342.1±119.1 175.35±45.11 1 768.4±402.1
EMT-2W 5 65.2±25.0□■ 255.43±39.51□■ 1 675.3±399.4△□
[1] Kim B, Peter B.Preventing and managing stress fractures in athletes[J].Phy Therapy Sport, 2005, 6: 171-180
[2] Arendt E, Agel J, Heikes C , et al. Stress injuries to bone in college athletes: a retrospective review of experience at a single institution[J]. Am J Sports Med, 2003,31(6):959-968
doi: 10.1177/03635465030310063601
[3] Chen YX, Li GP, Yao HE . A simulative study of exercise-induced bone fatigue[J]. Zhongguo Yun Dong Yi Xue Za Zhi, 2003,22(4):324-330
doi: 10.3969/j.issn.1000-6710.2003.04.001
陈佑学, 李国平, 姚鸿恩 . 运动性骨疲劳的模拟实验研究[J]. 中国运动医学杂志, 2003,22(4):324-330
doi: 10.3969/j.issn.1000-6710.2003.04.001
[4] Yoshikawa T, Mori S, Santiesteban AJ , et al.The effects of muscle fatigue on bone strain[J].J Exp Biol, 1994, 188: 217-233
doi: 10.1046/j.1420-9101.1994.7020247.x pmid: 7964380
[5] Yang WY, Liang R, Yang M , et al. Effect of Chinese decoction of "invigorating the spleen and regulating the flow of qi"on energy metabolism in rat skeletal muscles[J]. Zhongguo Yun Dong Yi Xue Za Zhi, 1994,13(1):28-31
杨维益, 梁嵘, 杨敏 , 等. 健脾理气法对骨骼肌能量代谢影响的研究[J]. 中国运动医学杂志, 1994,13(1):28-31
[6] Li GP, Zhang SD, Chen G , et al. Radiographic and histologic analyses of stress fracture in rabbit tibias[J]. Am J Sports Med, 1985,13(5):285-294
doi: 10.1177/036354658501300501 pmid: 4051084
[7] Tian QJ . Experimental research of preventing and treating of tibial stress injury by external using traditional Chinese medicine[J]. Chengdu Zhong Yi Yao Da Xue Xue Bao, 2000,23(4):29-30
doi: 10.3969/j.issn.1004-0668.2000.04.012
田绮俊 . 外用中药防治胫骨应力损伤的实验研究[J]. 成都中医药大学学报, 2000,23(4):29-30
doi: 10.3969/j.issn.1004-0668.2000.04.012
[8] Zhou CJ, Wang XH, Zhu XH , et al. Effect of "Yiqi Huayu" to the internal energy on part of the fracture and to the content of Ca, P, Mn and Mg on fracture callus in rabbits[J]. Anhui Zhong Yi Xue Yuan Xue Bao, 1988,7(2):47-50
周重建, 王绪辉, 朱显华 , 等. 益气化淤对家兔骨折断端细胞内能及骨痂中钙、磷、锰、镁含量的影响[J]. 安徽中医学院学报, 1988,7(2):47-50
[9] Delmas PD. Biochemical markers of bone turnover in osteoporosis. In: Riggs BL, Melton LJ. Osteoporosis: etiology, diagnostics and management[M]. New York: Raven Press,1988: 297-316
[10] Wang DW, Gao XY, Li FM , et al. Effect of Eucommia ulmoides Oliv. extracts on the proliferation of osteoblast-like cells UMR106[J]. Zhong Yao Yao Li Yu Lin Chuang, 2000,16(4):24-26
王大为, 高晓燕, 李发美 , 等. 杜仲对成骨样细胞增殖的作用[J]. 中药药理与临床, 2000,16(4):24-26
[11] Wang YD, Liu XQ . Effect of Astragalus on the proliferation and differentiation of osteoblasts in vitro[J]. Hua Xia Yi Xue, 2004,17(3):306-308
doi: 10.3969/j.issn.1008-2409.2004.03.005
王永东, 刘晓青 . 中药黄芪对成骨细胞体外成骨能力的观察[J]. 华夏医学, 2004,17(3):306-308
doi: 10.3969/j.issn.1008-2409.2004.03.005
[12] Zhang N, Liu SW, Han FY , et al. Effects of tonify-kidney and activate-blood Chinese medicine on proliferation of rat's osteoblasts in vitro[J]. Zhongguo Gu Shang, 2004,17(11):659-661
doi: 10.3969/j.issn.1003-0034.yyyy.nn.zzz
张宁, 刘世巍, 韩凤岳 , 等. 补肾活血中药对体外培养成骨细胞增殖的影响[J]. 中国骨伤, 2004,17(11):659-661
doi: 10.3969/j.issn.1003-0034.yyyy.nn.zzz
[13] Nian H, Xu LL, Ma MH , et al. Prevention of bone loss by aqueous extract of Epimedii sagittatum in an ovariectomized rat model of osteoporosis[J]. Zhong Xi Yi Jie He Xue Bao, 2006,4(6):628-633
年华, 徐玲玲, 马明华 , 等. 箭叶淫羊藿对去卵巢大鼠骨量丢失的保护作用[J]. 中西医结合学报, 2006,4(6):628-633
[14] Seibel MJ, Robins SP, Bilezikian JP. Dynamics of bone and cartilage metabolism(2nd ed)[M]. San Diego, CA: Academic Press, 2006,( 2nd ed):201-212
[15] Qian FL, Zeng FH, Feng WQ . Effects of Chinese herbal preparation for invigorating kidney on function of hypothalamic-pituitary-gonad axis in rats[J]. Zhongguo Yun Dong Yi Xue Za Zhi, 2005,24(5):571-575
doi: 10.3969/j.issn.1000-6710.2005.05.013
钱风雷, 曾繁辉, 冯炜权 . 补肾中药对运动大鼠下丘脑-垂体-性腺轴功能的调节作用[J]. 中国运动医学杂志, 2005,24(5):571-575
doi: 10.3969/j.issn.1000-6710.2005.05.013
[16] Miki Y, Suzuki T, Hatori M , et al. Effects of aromatase inhibitors on human osteoblast and osteoblast-like cells: a possible androgenic bone protective effects induced by exemestane[J]. Bone, 2007,40(4):876-887
doi: 10.1016/j.bone.2006.11.029
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