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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (10): 1024-1028.doi: 10.3736/jcim20081007

• Original Experimental Research • Previous Articles     Next Articles

Effects of traditional Chinese herbal medicine on the neurobehavioral manifestations and the activity of dopamine D2 receptor in corpora striatum of rats with levodopa-induced dyskinesias

Can-xing Yuan1(), Qing Ye1, Jie Wang1, Yan Zhang2, Chong-gang Yuan2   

  1. 1. Department of Neurology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. Laboratory of Physiology, School of Life Science, East China Normal University, Shanghai 200062, China
  • Received:2008-03-14 Online:2008-10-20 Published:2008-10-15

Objective: 

To explore the effect of traditional Chinese herbal medicine (TCM) for nourishing liver and kidney, clearing meridians and removing toxic substances, on the neurobehavioral manifestations and the activity of the dopamine D2 receptor in rat with levodopa-induced dyskinesias (LID).


Methods: 

The rat model of Parkinson's disease (PD) was established by injecting 6-hydroxydopamine (6-OHDA) into right substantia nigra of brain, then, the model of LID in rat was produced by injecting levodopa (LD) and benserazide for 4 weeks. The rats were divided into normal control group, 4-week LD treated group, 4-week LD plus TCM treated group, 8-week LD treated group, and 8-week LD plus TCM treated group, and the effect of the TCM on neurobehavioral manifestations was observed. The radioligand binding assay (RLBA) and Scatchard drawing were used to measure the maximal binding capacity of receptor (Bmax) and equilibrium dissociation constant (KD) of the dopamine D2 receptor in corpora striatum.


Results: 

Compared with the 4-week LD treated group and 8-week LD treated group, TCM could decrease abnormal involuntary movement scores of the rats with LID; the RLBA revealed that the dopamine D2 receptor Bmax significantly increased (P<0.05, P<0.01) and the KD significantly decreased (P<0.05).


Conclusion: 

TCM can improve the activity of the dopamine D2 receptor and relieve the symptoms of LID.

Key words: levodopa-induced dyskinesias, traditional Chinese herbal medicine, dopamine D2 receptor, neurobehavioral manifestations, rats

CLC Number: 

  • R747

Figure 1

Effects of TCM on the scores of AIM in rats with LID **P<0.01, vs 0-week; △△P<0.01, vs 8-week LD treated group; ▲▲P<0.01, vs 4-week LD treated group."

Table 1

Effects of TCM on activity of dopamine D2 receptor in rats with LID (x±s)"

Group n Bmax (fmol/mg) KD (nmol/L)
Lesion side Uninjured side Lesion side Uninjured side
Normal control 6 1 286.10±162.43 1 237.36±149.19 1.020±0.126 0.999±0.135
8-week LD treated 6 531.41±51.66** 1 075.24±89.82 1.592±0.064** 1.268±0.083
8-week LD+TCM treated 6 776.54±100.39**△△ 1 204.04±164.41 1.059±0.057△△ 1.075±0.130
4-week LD treated 6 833.01±131.86** 1 138.61±163.94 1.473±0.059** 1.071±0.200
4-week LD+TCM treated 6 976.99±82.25** 1 240.38±159.69 0.898±0.058*▲▲ 0.980±0.157
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