Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (4): 363-367.doi: 10.3736/jcim20100411

• Original Experimental Research • Previous Articles     Next Articles

Immunoregulatory function of Radix Glycyrrhizae polysaccharide in tumor-bearing mice

 Xiao-bing Lia,b, Xiao-juan Heb, Biao Liub, Li Xuc, Da-hong Jud, Miao Jiangb, Ai-ping Lub   

  1. a Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    b Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China
    c College of Life Science,Hubei University, Wuhan 430062, Hubei Province, China
    d College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
    e Institute of Basic Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • Received:2009-12-16 Accepted:2010-02-08 Online:2010-04-20 Published:2010-04-15
  • Contact: Ai-ping Lu


To observe the effects of Radix Glycyrrhizae polysaccharide on regulatory T cells (Treg) in spleen and lymphocyte transformation ratio in tumor-bearing mice so as to explore the mechanisms of its immunoregulatory function.

Fifty BALB/c mice were randomly divided into normal group, untreated group, cyclophosphamide group, Radix Glycyrrhizae polysaccharide group and Radix Glycyrrhizae polysaccharide plus cyclophosphamide group. Except normal group, mice were subcutaneously implanted H22 tumor cells in the right axillary region. After 24 h, mice in normal and untreated group were subcutaneously injected with physiological saline, while mice in the cyclophosphamide group were intraperitoneally injected with cyclophosphamide and mice in Radix Glycyrrhizae polysaccharide group were subcutaneously injected with polysaccharide. Fourteen days later, Treg cells of spleen were detected by flow cytometry and lymphocyte transformation ratio was detected by methyl thiazolyl tetrazolium method.

The proportion of Treg cells was significantly higher in the untreated group than in the normal group, and was lower in the Radix Glycyrrhizae polysaccharide group than in the untreated group (P<0.01). Lymphocyte transformation ratio in the Radix Glycyrrhizae polysaccharide group was higher than that in the cyclophosphamide group. There was no interaction between Radix Glycyrrhizae polysaccharide and cyclophosphamide.

Radix Glycyrrhizae polysaccharide can regulate the cellular immunity disorders of tumor-bearing mice by decreasing proportion of Treg cells and increasing spleen lymphocyte transformation ratio.

Key words: Glycyrrhiza, T-lymphocytes, Regulatory, Liver neoplasms, Experimental, Mice

Figure 1

Effects of Radix Glycyrrhizae polysaccharide on spleen index of tumor bearing mice *P<0.05, **P<0.01, vs normal group; ▲P<0.05, vs CTX group. Data were represented as $\bar{x}$±s, n=6."

Figure 2

Effects of Radix Glycyrrhizae polysaccharide on spleen lymphocyte transformation ratio *P<0.05, **P<0.01, vs normal group; △P<0.05, vs untreated group; ▲P<0.05, ▲▲P<0.01, vs CTX group. Data were represented as $\bar{x}$±s, n=6."

Figure 3

Effects of Radix Glycyrrhizae polysaccharide on ratio of CD4+CD25+Foxp3+ and CD4+ T cells *P<0.05, **P<0.01, vs normal group; △△P<0.01, vs untreated group; ▲▲P<0.01, vs CTX group. Data were represented as $\bar{x}$±s, n=6."

[1] He ZG. Traditional Chinese medicine[M]. Beijing: People's Medical Publishing House, 1996: 136
贺志光 . 中医学[M]. 北京: 人民卫生出版社, 1996: 136
[2] Cheng A, Wan F, Jin Z, Wang J, Xu X . Nitrite oxide and inducible nitric oxide synthase were regulated by polysaccharides isolated from Glycyrrhiza uralensis Fisch[J]. J Ethnopharmacol, 2008,118(1):59-64
doi: 10.1016/j.jep.2008.03.002
[3] Cheng A, Wan F, Wang J, Jin Z, Xu X . Macrophage immunomodulatory activity of polysaccharides isolated from Glycyrrhiza uralensis Fish[J]. Int Immunopharmacol, 2008,8(1):43-50
doi: 10.1016/j.intimp.2007.10.006
[4] Cheng AW, Jin ZY, Wan FC, Xu XM . Effects of Glycyrrhiza polysaccharide on cytochemistry and intracellular endoenzyme of mice peritoneal macrophages[J]. Shi Pin Yu Sheng Wu Ji Shu Xue Bao, 2008,27(1):76-79
程安玮, 金征宇, 万发春, 徐学明 . 甘草多糖对小鼠腹腔巨噬细胞化学成分及胞内酶的影响[J]. 食品与生物技术学报, 2008,27(1):76-79
[5] Gong FL. Medical immunology[M]. Beijing: Science Press, 2003
龚非力 . 医学免疫学[M]. 北京: 科学出版社, 2003
[6] Matar P, Rozados VR, González AD, Dlugovitzky DG, Bonfil RD, Scharovsky OG . Mechanism of antimetastatic immunopotentiation by low-dose cyclophosphamide[J]. Eur J Cancer, 2000,36(8):1060-1066
doi: 10.1016/S0959-8049(00)00044-7
[7] Di Paolo NC, Tuve S, Ni S, Hellstrom KE, Hellstrom I, Lieber A . Effect of adenovirus-mediated heat shock protein expression and oncolysis in combination with low-dose cyclophosphamide treatment on antitumor immune responses[J]. Cancer Res, 2006,66(2):960-969
doi: 10.1158/0008-5472.CAN-05-2388
[8] Tian WW, Li O, Huang YH, Deng DC, Wang QM . Effect of Buxu Huayu Prescription on myeloid hematopoiesis in tumor-bearing mice after combined chemotherapy[J]. Zhong Yao Xin Yao Yu Lin Chuang Yao Li, 2003,14(1):3-6
田卫卫, 李偶, 黄映红, 邓道昌, 王庆苗 . 补虚化瘀方对联合化疗荷瘤小鼠骨髓造血功能的影响[J]. 中药新药与临床药理, 2003,14(1):3-6
[9] Duan QH, Wang G, Hu B . Research progress in CD4+ CD25high FOXP3+ regulatory T cells and tumor[J]. Xian Dai Zhong Liu Yi Xue, 2009,17(5):981-984
段巧虹, 王刚, 胡冰 . CD4+CD25high FOXP3+调节性T细胞与肿瘤的研究进展[J]. 现代肿瘤医学, 2009,17(5):981-984
[10] Ghiringhelli F, Larmonier N, Schmitt E, Parcellier A, Cathelin D, Garrido C, Chauffert B, Solary E, Bonnotte B, Martin F . CD4+CD25+ regulatory T cells suppress tumor immunity but are sensitive to cyclophosphamide which allows immunotherapy of established tumors to be curative[J]. Eur J Immunol, 2004,34(2):336-344
doi: 10.1002/(ISSN)1521-4141
[11] Lutsiak ME, Semnani RT, De Pascalis R, Kashmiri SV, Schlom J, Sabzevari H . Inhibition of CD4+25+ T regulatory cell function implicated in enhanced immune response by low-dose cyclophosphamide[J]. Blood, 2005,105(7):2862-2868
doi: 10.1182/blood-2004-06-2410
[12] Mihalyo MA, Doody AD , McAleer JP, Nowak EC, Long M, Yang Y, Adler AJ . In vivo cyclophosphamide and IL-2 treatment impedes self-antigen-induced effector CD4 cell tolerization: implications for adoptive immunotherapy[J]. J Immunol, 2004,172(9):5338-5345
doi: 10.4049/jimmunol.172.9.5338
[13] Fontenot JD, Gavin MA, Rudensky AY . Foxp3 programs the development and function of CD4+CD25+ regulatory T cells[J]. Nat Immunol, 2003,4(4):330-336
doi: 10.1038/ni904
[1] Victor Abou Nehmi Filho, Gilson Masahiro Murata, Ruan Carlos Macêdo de Moraes, Gabriely Cristina Alves Lima, Danielle Araujo de Miranda, Katrin Radloff, Raquel Galvão Figuerêdo Costa, Joyce de Cassia Rosa de Jesus, Jéssica Alves De Freitas, Nayara Izabel Viana, Ruan Pimenta, Katia Ramos Moreira Leite, José Pinhata Otoch, Ana Flávia Marçal Pessoa. A novel supplement with yeast β-glucan, prebiotic, minerals and Silybum marianum synergistically modulates metabolic and inflammatory pathways and improves steatosis in obese mice. Journal of Integrative Medicine, 2021, 19(5): 439-450.
[2] Hao Gou, Ruo-chen Huang, Yong-hua Su, Wei Li. Design of dual targeting immunomicelles loaded with bufalin and study of their anti-tumor effect on liver cancer. Journal of Integrative Medicine, 2021, 19(5): 408-417.
[3] Yi Ran Hu, San Li Xing, Chuan Chen, Ding Zhu Shen, Jiu Lin Chen. Tiaoxin Recipe, a Chinese herbal formula, inhibits microRNA-34a expression in the APPswe/PS1ΔE9 mouse model of Alzheimer's disease. Journal of Integrative Medicine, 2019, 17(6): 404-409.
[4] Bérénice Colleville, Nicolas Perzo, Guillaume Avinée, Anaïs Dumesnil, Frederic Ziegler, Paul Billoir, Hélène Eltchaninoff, Vincent Richard, Eric Durand. Impact of high-fat diet and vitamin D3 supplementation on aortic stenosis establishment in waved-2 epidermal growth factor receptor mutant mice. Journal of Integrative Medicine, 2019, 17(2): 107-114.
[5] Bhesh Raj Sharma, Dong Wook Kim, Dong Young Rhyu. Korean Chungtaejeon tea extract attenuates weight gain in C57BL/6J-Lep ob/ob mice and regulates adipogenesis and lipolysis in 3T3-L1 adipocytes. Journal of Integrative Medicine, 2017, 15(1): 56-63.
[6] Shi-li Jiang, Xu-dong Hu, Ping Liu. Immunomodulation and liver protection of Yinchenhao decoction against concanavalin A-induced chronic liver injury in mice. Journal of Integrative Medicine, 2015, 13(4): 262-268.
[7] Xuanbin Wang, Ning Wang, Fan Cheung, Lixing Lao, Charlie Li, Yibin Feng. Chinese medicines for prevention and treatment of human hepatocellular carcinoma: Current progress on pharmacological actions and mechanisms. Journal of Integrative Medicine, 2015, 13(3): 142-164.
[8] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[9] Md. Asadujjaman, Ahmed Ullah Mishuk, Md. Aslam Hossain, Utpal Kumar Karmakar. Medicinal potential of Passiflora foetida L. plant extracts: Biological and pharmacological activities. Journal of Integrative Medicine, 2014, 12(2): 121-126.
[10] Marjan Nassiri-Asl, Taghi Naserpour Farivar, Esmail Abbasi, Hamid Reza Sadeghnia, Mehdi Sheikhi, Mina Lotfizadeh, Parisa Bazahang. Effects of rutin on oxidative stress in mice with kainic acid-induced seizure. Journal of Chinese Integrative Medicine, 2013, 11(5): 337-342.
[11] Md. Khirul Islam, Imran Mahmud, Sanjib Saha, Asit Baron Sarker, Himangsu Mondal, A.S.M. Monjur-Al-Hossain, Md. Anisuzzman. Preliminary pharmacological evaluation of Alocasia indica Schott tuber. Journal of Chinese Integrative Medicine, 2013, 11(5): 343-351.
[12] Sanjib Saha, Faroque Hossain, Md. Anisuzzman, Md. Khirul Islam. Pharmacological evaluation of Musa seminifera Lour. Fruit. Journal of Chinese Integrative Medicine, 2013, 11(4): 253-261.
[13] Sunil A. Nirmal, Jayashri M. Ingale, Shashikant R. Pattan, Sanjay B. Bhawar. Amaranthus roxburghianus root extract in combination with piperine as a potential treatment of ulcerative colitis in mice. Journal of Chinese Integrative Medicine, 2013, 11(3): 206-212.
[14] Samuel Joshua Pragasam, Vachana Murunikara, Evan Prince Sabina, Mahaboobkhan Rasool. Antiperoxidative potential of p-coumaric acid, a common dietary phenol, in adjuvant-induced arthritis in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 932-938.
[15] Dong-mei Zhang, Li-xia Lou, Ai-ming Wu, Xi-ying Lü, Zhao-juan Hu, Yan-hong Zhang, Hong-fang Liu. Effects of Astragalus membranaceus and Potentilla discolor mixture on insulin resistance and its related mRNA expressions in KKAy mice with type 2 diabetes. Journal of Chinese Integrative Medicine, 2012, 10(7): 821-826.
Full text



[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239