Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (5): 391-395.doi: 10.3736/jcim20050515

• Original Experimental Research • Previous Articles     Next Articles

Protective and therapeutic effects of Shugan Lifei Recipe on rats with asthma under stress and the mechanisms

Yao Tong1,Ning-xia Zhang2,Hui-juan Chen3,Li-qiang Ni4   

  1. 1. School of Chinese Medicine, University of Hong Kong, Hong Kong, China
    2. Department of Rehabilitation, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China
    3. Basic Medical College, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China
    4. Shanghai Institute of Medical Information, Shanghai 200063, China
  • Received:2005-04-14 Online:2005-09-20 Published:2005-09-20
  • Contact: Yao Tong E-mail:tongyao@hku.hk

Objective

To explore the influence of psychological factor on asthma, the protective and therapeutic effects of Shugan Lifei Recipe on rats with asthma under stress and the mechanisms.

Methods

Allergy and stress-induced asthma models were established in rats by giving ovalbumin and restraining stress. Radioimmunoassay, enzyme linked immunosorbent assay, in situ hybridization and immunohistrochemical method were used to detect plasma corticosterone, IL-4, IFN-γ, expression of glucocorticoid receptor (GR) mRNA in hippocampus and adrenocorticotropic hormone corticoliberin (CRH) positve neuron in hypothalamus. Optical and electron microscopes were used to observe the morphological changes of pulmonary hilar and hippocampal tissues.

Results

Shugan Lifei Recipe (SGLFR) could reduce plasma corticosterone, decrease CRH positive neurons in paraventricular nucleus of hypothalamus, up-regulate the expression of GR mRNA in hippocampus and reduce the injury of hippocampal neuron. SGLFR had certain inhibitive effect on hyperfunction of hypothalamo-pituitary-adrenal cortex (HPA) axis in rats with asthma under stress and could also relieve the pathological changes of the pulmonary hilar tissue. The level of plasma IFN-γ was increased while the level of plasma IL-4 was decreased in SGLFR-treated group.

Conclusion

The mechanism of SGLFR in treating the rats with asthma under stress is probably to regulate the hyperfunction of HPA axis and the disorder of immuo-system.

Key words: Stress, Asthma, Dispersing stagnated liver qi and regulating lung qi

CLC Number: 

  • R562.65

Tab 1

Comparison of plasma corticosterone in different groups ($\bar{x}$±s, mg/L)"

Group n Plasma corticosterone
Normal control 8 235.27±46.64
Allergy-induced asthma model 8 323.32±100.40*
Stress-induced model 8 532.48±115.28**
Allergy and stress-induced
asthma model
8 569.89±72.32△△
Dexamethasone-treated 8 392.43±112.90▲▲
LFR-treated 8 528.60±130.72■■
SGLFR-treated 8 309.50±46.52▲▲□□■

Tab 2

Comparison of expression of GR mRNA in hippocampus and number of CRH positive neurons in paraventricular nucleus of hypothalamus in different groups ($\bar{x}$±s )"

Group n GR mRNA (mg/L) CRH positive neuron (/7×104 μm2)
Normal control 3 7.67±0.52 14.33±3.07
Allergy-induced asthma model 3 6.00±0.63** 37.83±7.25**
Stress-induced model 3 3.67±0.52** 174.50±4.68**
Allergy and stress-induced asthma model 3 3.33±0.52△△ 174.33±3.93△△
Dexamethasone-treated 3 3.67±0.52 61.33±6.56▲▲
LFR-treated 3 4.00±0.89 170.17±3.18
SGLFR-treated 3 5.33±0.52▲▲□□■■ 108.17±16.18▲▲□□■■

Tab 3

Comparison of plasma IL-4 and IFN-γ in different groups ($\bar{x}$±s )"

Group n IL-4 (ng/L) IFN-γ (ng/L)
Normal control 8 37.53±5.20 79.44±25.30
Allergy-induced asthma model 8 58.90±18.13** 55.19±13.31**
Stress-induced model 8 54.07±9.29** 43.26±8.48**
Allergy and stress-induced asthma model 8 82.91±23.07△△ 37.07±5.65△△■
Dexamethasone-treated 8 42.68±2.41▲▲ 42.44±7.14
LFR-treated 8 40.51±2.79▲▲ 51.63±15.72
SGLFR-treated 8 39.82±6.48▲▲ 62.31±26.99▲▲□■
[1] Sousa N, Lukoyanov NV, Madeira MD , et al. Reorganization of the morphology of hippocampal neurites and synapses after stress-induced damage correlates with behavioral improvement[J]. Neuroscience, 2000,97(2):253-266
doi: 10.1016/S0306-4522(00)00050-6
[2] Feldman S, Weidenfeld J . Electrical stimulation of the dorsal hippocampus caused a long lasting inhibition of ACTH and adrenocortical responses to photic stimuli in freely moving rats[J]. Brain Res, 2001,911(1):22-26
doi: 10.1016/S0006-8993(01)02538-0
[3] Makino S, Smith MA, Gold PW . Increased expression of corticotropin-releasing hormone and vasopressin messenger ribonucleic acid(mRNA) in the hypothalamic paraventricular nucleus during repeated stress: association with reduction in glucocorticoid receptor mRNA levels[J]. Endocrinology, 1995,136(8):3299-3309
doi: 10.1210/endo.136.8.7628364
[4] 钱瑞琴, 张春英, 杨宇 . 疏肝中药对应激小鼠免疫功能影响的对比研究[J]. 中成药, 2000,22(9):645-647
[5] 胡盛珊, 王大元, 邱萍 . 枳实有效成分的药理活性比较[J]. 中草药, 1994,25(8):419-421
[6] 尹浩军 . 肝气郁结证的实验研究近况[J]. 国医论坛, 1996,11(2):45-48
[7] 李峰, 杨维益, 梁山荣 . 从中医学看肝脏调节应激反应的作用[J]. 北京中医药大学学报, 1998,21(1):20-22
[8] 严灿, 邓中炎, 吴伟康 . 从心理应激理论研究中医肝主疏泄脏象本质[J]. 中医杂志, 2001,42(1):8-10
doi: 10.3321/j.issn:1001-1668.2001.01.102
[9] 吴东辉 . 即刻早期基因与心理应激[J]. 国外医学·精神病学分册, 2001,28(2):119-122
[10] 李泽桂, 陆振山, 蔡文琴 . 应激与神经肽[J]. 第三军医大学学报, 1999,21(9):693-696
[11] Herman JP, Dolgas CM, Carlson SL . Ventral subiculum regulates hypothalamo-pituitary-adrenocortical and behavioural responses to cognitive stressors[J]. Neuroscience, 1998,86(2):449-459
doi: 10.1016/S0306-4522(98)00055-4
[12] 朱晓蔓, 朱子涛, 温传俊 , 等. 海马内NA能神经损毁对抗急性低氧诱发皮质酮分泌[J]. 生理学报, 1999,51(3):356-360
doi: 10.3321/j.issn:0371-0874.1999.03.021
[13] Herman JP, Larson BR . Differential regulation of forebrain glutamic acid decarboxylase mRNA expression by aging and stress[J]. Brain Res, 2001,912(1):60-66
doi: 10.1016/S0006-8993(01)02641-5
[14] Herman JP, Spencer R . Regulation of hippocampal glucocorticoid receptor gene transcription and expression in vivo[J]. J Neurosci, 1998,18(18):7462-7473
doi: 10.1523/JNEUROSCI.18-18-07462.1998
[15] 严进, 汤淑萍, 路长林 , 等. 睫状神经营养因子对应激引起海马CA3神经元损伤的作用[J]. 解剖学杂志, 1999,22(2):99-104
[16] McIntosh LJ, Sapolsky RM . Glucocorticoids may enhance oxygen radical-mediated neurotoxicity[J]. Neurotoxicology, 1996,17(3-4):873-882
doi: 10.1016/0893-133X(95)00235-6 pmid: 9086511
[17] Von Hertzen LC . Maternal stress and T-cell differentiation of the developing immune system: possible implications for the development of asthma and atopy[J]. J Allergy Clin Immunol, 2002,109(6):923-928
doi: 10.1067/mai.2002.124776
[18] 李义, 赵玉军, 吴长德 . 应激与免疫[J]. 动物医学进展, 1999,20(3):32-34
[1] Célia Cristina Malaguti Figueiredo, Amanda da Costa Gomes, Filipe Oliveira Granero, João Luiz Bronzel Junior, Luciana Pereira Silva, Valdecir Farias Ximenes, Regildo Márcio Gonçalves da Silva. Antiglycation and antioxidant activities of the crude extract and saponin fraction of Tribulus terrestris before and after microcapsules release. Journal of Integrative Medicine, 2022, 20(2): 153-162.
[2] Mohamed S. Othman, Sofian T. Obeidat, Amal H. Al-Bagawi, Mohamed A. Fareid, Alaa Fehaid, Ahmed E. Abdel Moneim. Green-synthetized selenium nanoparticles using berberine as a promising anticancer agent#br#
#br#
. Journal of Integrative Medicine, 2022, 20(1): 65-72.
[3] Shuai Chen, Jin Hu, Deng-cheng Lu, Hong-yi Liu, Shan-shan Wei. Metabolomic characteristics of spontaneously hypertensive rats under chronic stress and the treatment effect of Danzhi Xiaoyao Powder, a traditional Chinese medicine formula. Journal of Integrative Medicine, 2022, 20(1): 73-82.
[4] Hao-cheng Zheng, Zi-rui Liu, Ya-lan Li, Yong-an Wang, Jing-wei Kong, Dong-yu Ge, Gui-ying Peng. Allium tuberosum alleviates pulmonary inflammation by inhibiting activation of innate lymphoid cells and modulating intestinal microbiota in asthmatic mice. Journal of Integrative Medicine, 2021, 19(2): 158-166.
[5] Olarewaju M. Oluba, Oghenerobor B. Akpor, Feyikemi D. Adebiyi, Sunday J. Josiah, Olayinka O. Alabi, Ayoola O. Shoyombo, Augustine O. Olusola. Effects of co-administration of Ganoderma terpenoid extract with chloroquine on inflammatory markers and antioxidant status in Plasmodium berghei-infected mice. Journal of Integrative Medicine, 2020, 18(6): 522-529.
[6] Leandro Lucena, Cristina Frange, Ana Claudia Amorim Pinto, Monica Levy Andersen, Sergio Tufik, Helena Hachul. Mindfulness interventions during pregnancy: A narrative review. Journal of Integrative Medicine, 2020, 18(6): 470-477.
[7] Louisa Sylvia, Emerson West, Allyson M. Blackburn, Carina Gupta, Eric Bui, Tara Mahoney, Geraldine Duncan, Edward C. Wright, Simon Lejeune, Thomas J. Spencer. Acceptability of an adjunct equine-assisted activities and therapies program for veterans with posttraumatic stress disorder and/or traumatic brain injury. Journal of Integrative Medicine, 2020, 18(2): 169-173.
[8] Gerli E.G. Herr, Fábio Goulart da Silva, Francisco José Cidral-Filho, Fabricia Petronilho, Lucinéia Gainski Danielski, Mariana Pereira de Souza Goldim, Afonso Shiguemi Inoue Salgado, Franciane Bobinski, Daniel Fernandes Martins, Eliane R. Winkelmann. Effects of the use of bioceramic wraps in patients with lower limb venous ulcers: A randomized double-blind placebo-controlled trial. Journal of Integrative Medicine, 2020, 18(1): 27-.
[9] Gaëtan Olivier Fankem, Michel Archange Fokam Tagne, Paul Aimé Noubissi, Angèle Foyet Fondjo, Idrice Kamtchouing, Adela Ngwewondo, Henri Wambe, Joseph Ngakou Mukam, René Kamgang. Antioxidant activity of dichloromethane fraction of Dichrocephala integrifolia in Salmonella typhi-infected rats. Journal of Integrative Medicine, 2019, 17(6): 438-445.
[10] Álisson de Carvalho Gonçalves, Einy Jéssika Siqueira Moreira, Guilherme Vannucchi Portari. Benfotiamine supplementation prevents oxidative stress in anterior tibialis muscle and heart. Journal of Integrative Medicine, 2019, 17(6): 423-429.
[11] Pranay Soni, Rajesh Choudhary, Surendra H. Bodakhe. Effects of a novel isoflavonoid from the stem bark of Alstonia scholaris against fructose-induced experimental cataract. Journal of Integrative Medicine, 2019, 17(5): 374-382.
[12] Fang-yuan Wang, Jian Jia, Huan-huan Song, Cheng-ming Jia, Chang-bo Chen, Jing Ma. Icariin protects vascular endothelial cells from oxidative stress through inhibiting endoplasmic reticulum stress. Journal of Integrative Medicine, 2019, 17(3): 205-212.
[13] Pádraic J.Dunne, Julie Lynch, Lucia Prihodova, Caoimhe O'Leary, Atiyeh Ghoreyshi, Sharee A. Basdeo, Donal J.Cox, Rachel Breen, Ali Sheikhi, Áine Carroll, Cathal Walsh, Geraldine McMahon, Barry White. Burnout in the emergency department: Randomized controlled trial of an attention-based training program. Journal of Integrative Medicine, 2019, 17(3): 173-180.
[14] Che Badariah Abd Aziz, Siti Qusyasyiah Ahmad Suhaimi, Hidani Hasim, Asma Hayati Ahmad, Idris Long, Rahimah Zakaria. Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed rats. Journal of Integrative Medicine, 2019, 17(1): 66-70.
[15] Lucky Legbosi Nwidu, Yibala Ibor Oboma. Telfairia occidentalis (Cucurbitaceae) pulp extract mitigates rifampicin-isoniazid-induced hepatotoxicity in an in vivo rat model of oxidative stress. Journal of Integrative Medicine, 2019, 17(1): 46-56.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[2] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[5] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[6] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[7] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[8] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[9] Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[10] Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022