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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (6): 675-680.doi: 10.3736/jcim20070615

• Original Experimental Research • Previous Articles     Next Articles

Protective effect of Huaxia shallot preparation on human umbilical vein endothelial cell injury induced by oxidized low density lipoprotein and its mechanism

Jie-mei Zhang1, Jie Guo1, Xin Tu2, Zhao-hong Shi1, Jian-jun Hao1, Yu-he Ke1, Jiang-feng Guan1, Juan-juan He1   

  1. 1. Unit of Cardiology,Institute of Integrative Medicine,Wuhan First Hospital,Wuhan,Hubei Province 430022,China
    2. Human Genome Research Cent,Life and Technology College,Huazhong University of Science and Technology,Wuhan,Hubei Province 430074,China
  • Online:2007-11-20 Published:2007-11-15

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Objective: To observe the protective effect of Huaxia shallot preparation on human umbilical vein endothelial cell (HUVEC) injury induced by oxidized low density lipoprotein (Ox-LDL) in vitro.Methods: Ox-LDL was prepared and identified, and HUVECs were cultured. After 2-hour intervention of different drugs and 24-hour following intervention of Ox-LDL, the number of HUVECs was observed by phase contrast optical microscope and the activity of the HUVECs was observed by methyl thiazolyl tetrazolium (MTT) technique. Superoxide dismutase (SOD) activity and nitric oxide (NO) content were assayed by respective kit. The protein expressions and mRNA levels of peroxisome proliferators activated receptor γ (PPAR-γ) and endothelial nitric oxide synthase (eNOS) were measured by western blot technique and reverse transcription polymerase chain reaction (RT-PCR).Results: Ox-LDL could increase the apoptosis rate of the HUVECs and decrease the NO release as compared with the blank control group (P<0.05). These effects induced by Ox-LDL were all significantly inhibited by Huaxia shallot preparation. It could up-regulate the protein expressions and mRNA levels of PPAR-γ and eNOS significantly (P<0.05). Huaxia shallot preparation could decrease the apoptosis rate of the HUVECs.Conclusion: Ox-LDL may be involved in the initiation and progression of atherosclerosis by injuring the endothelial cells directly and may cause the endothelial dysfunction. Huaxia shallot preparation can protect against Ox-LDL induced endothelial cell injury by up-regulating the protein expressions and mRNA levels of PPAR-γ and eNOS. It suggests that Huaxia shallot preparation may play a role in the prevention and treatment of cardiovascular disease.

Key words: atherosclerosis, endothelium, low-density lipoproteins, nitric-oxide synthase ;peroxisomes, alli-um schoenoprasum

CLC Number: 

  • R972.6

Figure 1

Shape characteristics of human umbilical vein endothelial cells in different groups (phase contrast optical microscope,×100)A: Blank control group; B: Ox-LDL-treated group; C: Huaxia shallot-treated group; D: Pingfu-treated group; E: Salvia dropping pill-treated group; F: Lipitor-treated group."

Table 1

Effects of different drugs on the viability of human umbilical vein endothelial cells impaired by Ox-LDL ($\bar{x}±S$)"

Group n Optical density value
Blank control 8 0.450±0.016
Ox-LDL-treated 8 0.213±0.014*
Huaxia shallot-treated 8 0.381±0.015*△
Pingfu-treated 8 0.418±0.017*△▲
Salvia dropping pill-treated 8 0.277±0.016*△▲□
Lipitor-treated 8 0.326±0.019*△▲□■

Table 2

Effects of different drugs on number of human umbilical vein endothelial cells, NO content and SOD activity ($\bar{x}±S$)"

Group n Cell number (1×105/ml) SOD (U/ml） NO (μmol/L)
Blank control 6 38.25±1.71 21.16±7.09 600.75±35.42
Ox-LDL-treated 6 25.00±1.63* 14.42±5.54 265.50±22.35*
Huaxia shallot-treated 6 31.00±0.82*△ 16.85±6.57 391.75±22.60*△
Pingfu-treated 6 34.50±1.29*△ 18.43±6.28 527.25±22.87*△▲
Salvia dropping pill-treated 6 28.25±0.96*△ 14.92±6.02 334.50±27.43*△▲□
Lipitor-treated 6 28.75±2.63*△ 15.01±5.83 335.50±30.54*△▲□

Table 3

Effects of different drugs on cell apoptosis rate tested by AnnexinV-PI method in different groups ($\bar{x}±S$, %)"

Group n Cell apoptosis rate
Blank control 6 8.7±1.5
Ox-LDL-treated 6 26.8±5.9*
Huaxia shallot-treated 6 11.4±3.2△
Pingfu-treated 6 10.6±2.9△
Salvia dropping pill-treated 6 19.1±4.7△
Lipitor-treated 6 16.5±3.8△

Figure 2

mRNA expressions of PPAR-γ and eNOS in human umbilical vein endothelial cellsA: PPAR-γ mRNA; B: eNOS mRNA; 0: Marker, 1: Blank control group; 2: Ox-LDL-treated group; 3: Huaxia shallot-treated group; 4: Pingfu-treated group; 5: Salvia dropping pill-treated group; 6: Lipitor-treated group."

Figure 3

Protein expressions of PPAR-γ and eNOS in human umbilical vein endothelial cells A: PPAR-γ protein; B: eNOS protein; 1: Blank control group; 2: Ox-LDL-treated group; 3: Huaxia shallot-treated group; 4: Pingfu-treated group; 5: Salvia dropping pill-treated group; 6: Lipitor-treated group."

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