Search JIM Advanced Search

Journal of Integrative Medicine ›› 2021, Vol. 19 ›› Issue (2): 158-166.doi: 10.1016/j.joim.2020.11.003

• Original Experimental Research • Previous Articles     Next Articles

Allium tuberosum alleviates pulmonary inflammation by inhibiting activation of innate lymphoid cells and modulating intestinal microbiota in asthmatic mice

Hao-cheng Zhenga, Zi-rui Liub, Ya-lan Lib, Yong-an Wangb, Jing-wei Kongb, Dong-yu Gea, Gui-ying Pengb   

  1. a School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
    b School of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, China
  • Received:2020-02-06 Accepted:2020-07-11 Online:2021-03-12 Published:2020-12-02
  • Contact: Gui-ying Peng E-mail:penggy@bucm.edu.cn

Objective
This study tests whether long-term intake of Allium tuberosum (AT) can alleviate pulmonary inflammation in ovalbumin (OVA)-induced asthmatic mice and evaluates its effect on the intestinal microbiota and innate lymphoid cells (ILCs).

Methods
BALB/c mice were divided into three groups: phosphate buffer saline, OVA and OVA + AT. The asthmatic murine model was established by sensitization and challenge of OVA in the OVA and OVA + AT groups. AT was given to the OVA + AT group by oral gavage from day 0 to day 27. On day 28, mice were sacrificed. Histopathological evaluation of lung tissue was performed using hematoxylin and eosin, and periodic acid-Schiff staining. The levels of IgE in serum, interleukin-5 (IL-5) and IL-13 from bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay. The ILCs from the lung and gut were detected by flow cytometry. 16S ribosomal DNA sequencing was used to analyze the differences in colon microbiota among treatment groups.

Results
We found that long-term intake of AT decreased the number of inflammatory cells from BALF, reduced the levels of IL-5 and IL-13 in BALF, and IgE level in serum, and rescued pulmonary histopathology with less mucus secretion in asthmatic mice. 16S ribosomal DNA sequencing results showed that AT strongly affected the colonic bacteria community structure in asthmatic mice, although it had no significant effect on the abundance and diversity of the microbiota. Ruminococcaceae and Desulfovibrionaceae were identified as two biomarkers of the treatment effect of AT. Moreover, AT decreased the numbers of ILCs in both the lung and gut of asthmatic mice.

Conclusion
The results indicate that AT inhibits pulmonary inflammation, possibly by impeding the activation of ILCs and adjusting the homeostasis of gut microbiota in asthmatic mice.

Key words: Allium tuberosum, Asthma, Innate lymphoid cell, Intestinal microbiota

[1] Oluwakemi Josephine Awakan, Sylvia Omonirume Malomo, Abdullahi Adeyinka Adejare, Adedoyin Igunnu, Olubunmi Atolani, Abiodun Humphrey Adebayo, Bamidele Victor Owoyele . Anti-inflammatory and bronchodilatory constituents of leaf extracts of Anacardium occidentale L. in animal models. Journal of Integrative Medicine, 2018, 16(1): 62-70.
[2] Bolleddula Jayaprakasam, Nan Yang, Ming-Chun Wen, Rong Wang, Joseph Goldfarb, Hugh Sampson, Xiu-Min Li. Constituents of the anti-asthma herbal formula ASHMITM synergistically inhibit IL-4 and IL-5 secretion by murine Th2 memory cells, and eotaxin by human lung fibroblasts in vitro. Journal of Chinese Integrative Medicine, 2013, 11(3): 195-205.
[3] Yi-tao Zhao , Xin-guang Zhang , Li Bai , Li-qing Li , Jian-er Yu . Effects of Chinese herbal medicine Pingchuan Formula on airway inflammation, interferon-γ and interleukin-4 in mice with asthma. Journal of Chinese Integrative Medicine, 2012, 10(7): 807-813.
[4] Ke-hua Shi, Yin-gen Wu, Bi-dan Xiong, Xiao Yu. Effects of medicinal extract for tonifying kidney to relieve asthma on glucocorticoid receptor expression in lung tissues of rats with bronchial asthma. Journal of Chinese Integrative Medicine, 2010, 8(8): 785-789.
[5] Wen Sun, Jian Yu, Yu-min Shi, Hao Zhang, Ying Wang, Bing-bing Wu. Effects of Cordyceps extract on cytokines and transcription factors in peripheral blood mononuclear cells of asthmatic children during remission stage. Journal of Chinese Integrative Medicine, 2010, 8(4): 341-346.
[6] Pei Wang, Li-ping Xu, Xi-hong Jin, Jing Yang, Ren-hui Liu, Xiu-juan Wang . Effects of a glucocorticoid on development of kidney deficiency syndrome in a rat model of asthma. Journal of Chinese Integrative Medicine, 2010, 8(1): 80-85.
[7] Ying Zhang , Huang-jin Tong, Jing-hua Yu , Peng-cheng Gu, Xin-sheng Fan, Hui-qin Xu. Effects of San'ao decoction and its analogous prescriptions on airway inflammation in mice with respiratory syncytial virus- and ovalbumin-induced asthma. Journal of Chinese Integrative Medicine, 2009, 7(4): 354-359.
[8] Hui-hua Zhu , Jian-er Yu , Xiao-feng Zhang, Yan-ping Chen, Hui-fang Pang, Guo-hua Wang, Yu Guan, Jie Wu. Effects of Pingchuan Recipe on airway remodeling in mice with bronchial asthma. Journal of Chinese Integrative Medicine, 2009, 7(3): 261-267.
[9] Ying-lan Zhang, Yin-gen Wu. Effects of Kechuanluo oral liquid on eosinophil apoptosis and its regulation factors in lung tissues of asthmatic mice. Journal of Chinese Integrative Medicine, 2008, 6(6): 600-605.
[10] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97.
[11] Ying Zhang, Rui-ming Zhang, Jing Chang, Ting-qian Li, Huan-hai Cui, Bing Mao, Guang-yuan Chen, Wen-fu Tang, Lei Wang, Su-zhen Huang. A randomized controlled trial of Yiqi Pingchuan Granule in treating acute attack of bronchial spasm due to asthma of qi deficiency and cold syndrome. Journal of Chinese Integrative Medicine, 2007, 5(1): 23-27.
[12] Hui-feng Shen, Yi-qun Li, Ruo-min Jin, He Li, Liang Min. An experimental serum pharmacological study on an application method in traditional Chinese medicine for treatment and prevention of asthma. Journal of Chinese Integrative Medicine, 2007, 5(1): 70-73.
[13] Fu-feng Li, Guo-gang Li, You-zhong Wu, Jing Li, Xiao-ying Zhang, Hui-fang Wang, Yi-qin Wang. Immunological mechanism of exfoliative tongue fur in children with asthma. Journal of Chinese Integrative Medicine, 2005, 3(6): 446-449.
[14] Li-qing Li, Li-li Huo, Xin-guang Zhang, Jian-er Yu. Progress in research on relationship between bronchial asthma and Th1/Th2 imbalance. Journal of Chinese Integrative Medicine, 2005, 3(5): 403-407.
[15] Yao Tong, Ning-xia Zhang, Hui-juan Chen, Li-qiang Ni. Protective and therapeutic effects of Shugan Lifei Recipe on rats with asthma under stress and the mechanisms. Journal of Chinese Integrative Medicine, 2005, 3(5): 391-395.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228