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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (3): 261-267.doi: 10.3736/jcim20090312

• Original Experimental Research • Previous Articles     Next Articles

Effects of Pingchuan Recipe on airway remodeling in mice with bronchial asthma

 Hui-hua Zhua, Jian-er Yub, Xiao-feng Zhangc, Yan-ping Chena, Hui-fang Pangd,Guo-hua Wangc, Yu Guanc, Jie Wub   

  1. a Department of Pediatrics, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
    b Department of Pediatrics, Shanghai Hospital of Traditional Chinese Medicine, Shanghai 200071, China
    c Central Laboratory, Shanghai Hospital of Traditional Chinese Medicine, Shanghai 200071, China
    d Department of Pathology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Received:2008-09-23 Accepted:2008-10-30 Online:2009-03-20 Published:2009-03-15

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Objective

To study the effects of Pingchuan Recipe, a compound traditional Chinese herbal medicine for treating bronchial asthma, on macrophage inflammatory protein-1α (MIP-1α) and immunoglobulin E (IgE) contents and CD86 expression in a mouse model of bronchial asthma, and to investigate the mechanism of Pingchuan Recipe in regulating airway remodeling in mice with bronchial asthma.
Methods

Fifty BALB/c mice were randomly divided into normal control group, untreated group, dexamethasone group, low-dose Pingchuan Recipe group and normal-dose Pingchuan Recipe group. Bronchial asthma in mice was induced by intra-abdominal injection of mixed ovalbumin and stimulation by inhaling 5% ovalbumin. Mice in the normal control group and the untreated group did not receive treatment, but mice in the other groups were administered intragastrically with 50 g/(kg·d) Pingchuan Recipe, 25 g/(kg·d) Pingchuan Recipe and 0.75 mg/(kg·d) dexamethasone, respectively. After consecutive treatment for 8 weeks, the MIP-1α contents in peripheral blood and bronchoalveolar lavage fluid (BALF) were measured by an avidin biotin complex-enzyme-linked immunosorbent assay (ABC-ELISA) system, and the IgE contents in serum and BALF were measured by ELISA method. Direct immunfluorescence-flow cytometry was used to detect the percentages of CD86 + and CD3 –CD86 + cells in peripheral blood and BALF. General histological changes of the lung tissue were observed by HE staining and collagen fiber staining 12 weeks after activation.
Results

Compared with the normal control group, the contents of MIP-1α, the percentages of CD86 +cells and CD3 –CD86 + cells in peripheral blood and BALF, and the contents of IgE in serum and BALF were raised significantly (P<0.01). The contents of MIP-1α, the percentages of CD86 + cells and CD3 –CD86 + cells in peripheral blood and BALF, and the contents of IgE in serum and BALF in the treated groups were lower than those in the untreated group (P<0.01). The contents of MIP-1α in peripheral blood and BALF and the contents of IgE in serum and BALF had no significant differences among the treated groups (P>0.05). The percentage of CD3 –CD86 + cells in peripheral blood in low-dose Pingchuan Recipe group was lower than that in the dexamethasone group. Compared with the untreated group, the collagen deposition in the wall of bronchiole in the treated groups was decreased.
Conclusion

Pingchuan Recipe can decrease the contents of MIP-1α, IgE and the expressions of CD86 in peripheral blood and BALF, which may be one of its mechanisms in improving chronic airway inflammation and inhibiting airway remodeling.

Key words: Bronchial asthma, Pingchuan Recipe, Airway remodeling, Mice

Table 1

Effects of Pingchuan Recipe on MIP-1α content in mice with bronchial asthma (x±s, ng/L)"

Group n MIP-1α
Blood BALF
Normal control 10 17.88±1.90 19.27±1.38
Untreated 10 45.24±3.64** 42.89±2.27**
Dexamethasone 10 29.82±3.59**△△ 17.52±1.27*△△
Low-dose Pingchuan Recipe 10 31.16±2.34**△△ 19.06±2.17△△
Normal-dose Pingchuan Recipe 10 30.20±2.87**△△ 18.20±1.45△△

Table 2

Effects of Pingchuan Recipe on percentages of CD86+ and CD3– CD86+ cells in mice with bronchial asthma (x±s, %)"

Group n Percentage of CD86+ cells Percentage of CD3–CD86+ cells
Blood BALF Blood BALF
Normal control 10 15.93±2.10 20.80±1.62 8.27±1.03 7.02±1.09
Untreated 10 36.16±3.55** 43.26±2.94** 18.50±1.51** 17.59±1.66**
Dexamethasone 10 18.63±2.81*△△ 18.82±1.51*△△ 7.24±1.12△△ 6.53±0.83△△
Low-dose Pingchuan Recipe 10 18.93±1.92**△△ 20.44±2.82△△ 8.57±1.23△△▲ 7.17±0.91△△
Normal-dose Pingchuan Recipe 10 17.12±1.15△△ 19.91±1.57△△ 7.58±0.80△△ 6.65±1.30△△

Table 3

Effects of Pingchuan Recipe on IgE content in mice with bronchial asthma (x±s, U/mL)"

Group n IgE
Serum BALF
Normal control 10 23.16±2.01 32.49±2.77
Untreated 10 177.08±7.64** 146.06±8.21**
Dexamethasone 10 37.92±2.59**△△ 28.53±3.22△△
Low-dose Pingchuan Recipe 10 40.25±3.15**△△ 29.93±2.90△△
Normal-dose Pingchuan Recipe 10 38.17±2.66**△△ 30.15±1.92△△

Figure 1

Pathological changes of lung tissue in different groups tested by HE staining (Light microscopy,×200) A: Normal control group; B: Untreated group; C: Dexamethasone group; D: Low-dose Pingchuan Recipe group; E: Normal-dose Pingchuan Recipe group."

Figure 2

Pathological changes of lung tissue in different groups tested by collagen fiber staining (Light microscopy,×200) A: Normal control group; B: Untreated group; C: Dexamethasone group; D: Low-dose Pingchuan Recipe group; E: Normal-dose Pingchuan Recipe group."

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