SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (3): 261-267.doi: 10.3736/jcim20090312

• Original Experimental Research • Previous Articles     Next Articles

Effects of Pingchuan Recipe on airway remodeling in mice with bronchial asthma

 Hui-hua Zhua, Jian-er Yub, Xiao-feng Zhangc, Yan-ping Chena, Hui-fang Pangd,Guo-hua Wangc, Yu Guanc, Jie Wub   

  1. a Department of Pediatrics, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
    b Department of Pediatrics, Shanghai Hospital of Traditional Chinese Medicine, Shanghai 200071, China
    c Central Laboratory, Shanghai Hospital of Traditional Chinese Medicine, Shanghai 200071, China
    d Department of Pathology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Received:2008-09-23 Accepted:2008-10-30 Online:2009-03-20 Published:2009-03-15

RichHTML

1

PDF (PC)

57

Like

Objective

To study the effects of Pingchuan Recipe, a compound traditional Chinese herbal medicine for treating bronchial asthma, on macrophage inflammatory protein-1α (MIP-1α) and immunoglobulin E (IgE) contents and CD86 expression in a mouse model of bronchial asthma, and to investigate the mechanism of Pingchuan Recipe in regulating airway remodeling in mice with bronchial asthma.
Methods

Fifty BALB/c mice were randomly divided into normal control group, untreated group, dexamethasone group, low-dose Pingchuan Recipe group and normal-dose Pingchuan Recipe group. Bronchial asthma in mice was induced by intra-abdominal injection of mixed ovalbumin and stimulation by inhaling 5% ovalbumin. Mice in the normal control group and the untreated group did not receive treatment, but mice in the other groups were administered intragastrically with 50 g/(kg·d) Pingchuan Recipe, 25 g/(kg·d) Pingchuan Recipe and 0.75 mg/(kg·d) dexamethasone, respectively. After consecutive treatment for 8 weeks, the MIP-1α contents in peripheral blood and bronchoalveolar lavage fluid (BALF) were measured by an avidin biotin complex-enzyme-linked immunosorbent assay (ABC-ELISA) system, and the IgE contents in serum and BALF were measured by ELISA method. Direct immunfluorescence-flow cytometry was used to detect the percentages of CD86 + and CD3 –CD86 + cells in peripheral blood and BALF. General histological changes of the lung tissue were observed by HE staining and collagen fiber staining 12 weeks after activation.
Results

Compared with the normal control group, the contents of MIP-1α, the percentages of CD86 +cells and CD3 –CD86 + cells in peripheral blood and BALF, and the contents of IgE in serum and BALF were raised significantly (P<0.01). The contents of MIP-1α, the percentages of CD86 + cells and CD3 –CD86 + cells in peripheral blood and BALF, and the contents of IgE in serum and BALF in the treated groups were lower than those in the untreated group (P<0.01). The contents of MIP-1α in peripheral blood and BALF and the contents of IgE in serum and BALF had no significant differences among the treated groups (P>0.05). The percentage of CD3 –CD86 + cells in peripheral blood in low-dose Pingchuan Recipe group was lower than that in the dexamethasone group. Compared with the untreated group, the collagen deposition in the wall of bronchiole in the treated groups was decreased.
Conclusion

Pingchuan Recipe can decrease the contents of MIP-1α, IgE and the expressions of CD86 in peripheral blood and BALF, which may be one of its mechanisms in improving chronic airway inflammation and inhibiting airway remodeling.

Key words: Bronchial asthma, Pingchuan Recipe, Airway remodeling, Mice

Table 1

Effects of Pingchuan Recipe on MIP-1α content in mice with bronchial asthma (x±s, ng/L)"

Group n MIP-1α
Blood BALF
Normal control 10 17.88±1.90 19.27±1.38
Untreated 10 45.24±3.64** 42.89±2.27**
Dexamethasone 10 29.82±3.59**△△ 17.52±1.27*△△
Low-dose Pingchuan Recipe 10 31.16±2.34**△△ 19.06±2.17△△
Normal-dose Pingchuan Recipe 10 30.20±2.87**△△ 18.20±1.45△△

Table 2

Effects of Pingchuan Recipe on percentages of CD86+ and CD3– CD86+ cells in mice with bronchial asthma (x±s, %)"

Group n Percentage of CD86+ cells Percentage of CD3–CD86+ cells
Blood BALF Blood BALF
Normal control 10 15.93±2.10 20.80±1.62 8.27±1.03 7.02±1.09
Untreated 10 36.16±3.55** 43.26±2.94** 18.50±1.51** 17.59±1.66**
Dexamethasone 10 18.63±2.81*△△ 18.82±1.51*△△ 7.24±1.12△△ 6.53±0.83△△
Low-dose Pingchuan Recipe 10 18.93±1.92**△△ 20.44±2.82△△ 8.57±1.23△△▲ 7.17±0.91△△
Normal-dose Pingchuan Recipe 10 17.12±1.15△△ 19.91±1.57△△ 7.58±0.80△△ 6.65±1.30△△

Table 3

Effects of Pingchuan Recipe on IgE content in mice with bronchial asthma (x±s, U/mL)"

Group n IgE
Serum BALF
Normal control 10 23.16±2.01 32.49±2.77
Untreated 10 177.08±7.64** 146.06±8.21**
Dexamethasone 10 37.92±2.59**△△ 28.53±3.22△△
Low-dose Pingchuan Recipe 10 40.25±3.15**△△ 29.93±2.90△△
Normal-dose Pingchuan Recipe 10 38.17±2.66**△△ 30.15±1.92△△

Figure 1

Pathological changes of lung tissue in different groups tested by HE staining (Light microscopy,×200) A: Normal control group; B: Untreated group; C: Dexamethasone group; D: Low-dose Pingchuan Recipe group; E: Normal-dose Pingchuan Recipe group."

Figure 2

Pathological changes of lung tissue in different groups tested by collagen fiber staining (Light microscopy,×200) A: Normal control group; B: Untreated group; C: Dexamethasone group; D: Low-dose Pingchuan Recipe group; E: Normal-dose Pingchuan Recipe group."

[1] Li LQ, Yu JE, Zhang XG, Min WF, Xia Y . Pingchuan Formula for children’s bronchial asthma: an observation of 30 cases[J]. Zhong Yi Er Ke Za Zhi, 2008,4(1):14-16
李利清, 虞坚尔, 张新光, 闵伟福, 夏英 . 平喘方治疗儿童支气管哮喘30例临床观察[J]. 中医儿科杂志, 2008,4(1):14-16
[2] Zhu HH, Yu JE, Zhang XF, Chen YP, Pang HF, Wang GH, Guan Y, Wu J . Experimental effects of “Pingchuan Decoction” on bronchial asthma macrophage inflammatory protein-1α and expression of CD86 in mice[J]. Shanghai Zhong Yi Yao Za Zhi, 2008,42(9):58-62
朱慧华, 虞坚尔, 张晓峰, 陈燕萍, 庞惠芳, 王国华, 管宇, 吴杰 . 平喘方对支气管哮喘模型小鼠巨噬细胞炎性蛋白-1α水平及CD86分子表达的实验研究[J]. 上海中医药杂志, 2008,42(9):58-62
[3] Li YK. Experimental methodology of traditional Chinese medicine pharmacology(2nd ed)[M]. Shanghai: Shanghai Scientific and Technical Publishers, 2006,( 2nd ed):38
李仪奎 . 中药药理实验方法学(第2版)[M]. 上海: 上海科技出版社, 2006,( 第2版):38
[4] Cheng XM, Wang CZ, Qian GS . Inhibitive effects of Rhizoma tripterygii on T lymphocytes activation and interleukin-5 mRNA expression in sensitized mice[J]. Zhonghua Nei Ke Za Zhi, 2001,40(5):342-343
程晓明, 王长征, 钱桂生 . 雷公藤抑制的致敏小鼠T淋巴细胞活化及白细胞介素-5 mRNA表达[J]. 中华内科杂志, 2001,40(5):342-343
[5] Li CC, Zhang WX, Chen XF, Xie LW, He QS, Hu XG, Lin J, Li MR, Wu RX, Zhang ZX . Effect of macrophage inflammatory protein-1α and its mRNA on airway inflammation of mouse asthma model[J]. Zhonghua Er Ke Za Zhi, 2004,42(4):90-93
李昌崇, 张维溪, 陈小芳, 谢丽微, 何秋莎, 胡晓光, 林剑, 李孟荣, 吴荣熙, 张正霞 . 巨噬细胞炎性蛋白1α及其mRNA在哮喘小鼠气道炎症中的作用[J]. 中华儿科杂志, 2004,42(4):90-93
[6] Sun JF. Technology of experimental animal[M]. Beijing: People’s Medical Publishing House,2001: 357
孙敬方 . 实验动物方法学[M]. 北京: 人民卫生出版社,2001: 357
[7] Qi HW . Asthma airway remodeling – challenge to study of bronchial asthma[J]. Di Si Jun Yi Da Xue Xue Bao, 2002,23(13):1153-1154
戚好文 . 哮喘气道重建——支气管哮喘研究中所面临的挑战[J]. 第四军医大学学报, 2002,23(13):1153-1154
[8] Li MH . New concept of asthma and treatment research progress[J]. Zhongguo Lin Chuang Yi Sheng, 2004,32(2):2-4
李明华 . 哮喘病的新概念和治疗研究进展[J]. 中国临床医生, 2004,32(2):2-4
[9] Xie BS, Li M . Relationship between airway remodeling and bronchial asthma[J]. Guo Wai Yi Xue Hu Xi Xi Tong Fen Ce, 1999,19(3):124-125,129
谢宝松, 李明 . 气道重建与支气管哮喘[J]. 国外医学呼吸系统分册, 1999,19(3):124-125,129
[10] Zhang WX, Li CC . Relationship between macrophage inflammatory protein-1α and bronchial asthma[J]. Guo Wai Yi Xue Er Ke Xue Fen Ce, 2002,29(2):65-67
张维溪, 李昌崇 . 巨噬细胞炎性蛋白-1α与支气管哮喘[J]. 国外医学儿科学分册, 2002,29(2):65-67
[11] Tamura N, Ishii N, Nakazawa M, Nagoya M, Yoshinari M, Amano T, Nakazima H, Minami M . Requirement of CD80 and CD86 molecules for antigen presentation by eosinophils[J]. Scand J Immunol, 1996,44(3):229-238
doi: 10.1046/j.1365-3083.1996.d01-303.x
[12] Huang ZY, Yang DD, Wu LN, Zheng GF, Zhou ML, Chen ZX, Liang ZY, Chen JY . Effects of Jiawei Shegan Mahuang Decoction on ultrastructural changes in lung tissues in guinea pigs with allergic asthma: observation by electron microscopy[J]. Zhong Yi Yao Yan Jiu, 1998,14(3):27-28
黄直炎, 杨冬娣, 吴玲霓, 郑高飞, 周名璐, 陈芝喜, 梁直英, 陈津岩 . 加味射干麻黄汤对过敏性哮喘豚鼠肺超微结构变化的电镜观察[J]. 中医药研究, 1998,14(3):27-28
[13] Polito AJ, Proud D . Epithelia cells as regulators of airway inflammation[J]. J Allergy Clin Immunol, 1998,102(5):714-718
doi: 10.1016/S0091-6749(98)70008-9
[14] Tillie-Leblond I, Hammad H, Desurmont S, Pugin J, Wallaert B, Tonnel AB, Gosset P . CC chemokines and interleukin-5 in bronchial lavage fluid from patients with status asthmaticus. Potential implication in eosinophil recruitment[J]. Am J Respir Crit Care Med, 2000,162(2 Pt 1):586-592
doi: 10.1164/ajrccm.162.2.9907014
[15] Chen WF. Medical immunology(3rd ed)[M]. Beijing: People’s Medical Publishing House, 2000,( 3rd ed):90-97
陈慰峰 . 医学免疫学(第3版)[M]. 北京: 人民卫生出版社, 2000,( 第3版):90-97
[16] Wang Q, Lin JT, Sun HT, Ye LY, Li J, Zhao WS . The study of T helper cell subsets and relationship to relative cytokines and ventilatory function in asthmatic patients[J]. Zhonghua Jie He He Hu Xi Za Zhi, 1999,22(3):147-150
王群, 林江涛, 孙洪涛, 叶丽亚, 李静, 赵武述 . 哮喘患者TH亚群的失衡及与相关细胞因子、肺通气功能改变的相关性研究[J]. 中华结核和呼吸杂志, 1999,22(3):147-150
[1] Victor Abou Nehmi Filho, Gilson Masahiro Murata, Ruan Carlos Macêdo de Moraes, Gabriely Cristina Alves Lima, Danielle Araujo de Miranda, Katrin Radloff, Raquel Galvão Figuerêdo Costa, Joyce de Cassia Rosa de Jesus, Jéssica Alves De Freitas, Nayara Izabel Viana, Ruan Pimenta, Katia Ramos Moreira Leite, José Pinhata Otoch, Ana Flávia Marçal Pessoa. A novel supplement with yeast β-glucan, prebiotic, minerals and Silybum marianum synergistically modulates metabolic and inflammatory pathways and improves steatosis in obese mice. Journal of Integrative Medicine, 2021, 19(5): 439-450.
[2] Hao Gou, Ruo-chen Huang, Yong-hua Su, Wei Li. Design of dual targeting immunomicelles loaded with bufalin and study of their anti-tumor effect on liver cancer. Journal of Integrative Medicine, 2021, 19(5): 408-417.
[3] Yi Ran Hu, San Li Xing, Chuan Chen, Ding Zhu Shen, Jiu Lin Chen. Tiaoxin Recipe, a Chinese herbal formula, inhibits microRNA-34a expression in the APPswe/PS1ΔE9 mouse model of Alzheimer's disease. Journal of Integrative Medicine, 2019, 17(6): 404-409.
[4] Bhesh Raj Sharma, Dong Wook Kim, Dong Young Rhyu. Korean Chungtaejeon tea extract attenuates weight gain in C57BL/6J-Lep ob/ob mice and regulates adipogenesis and lipolysis in 3T3-L1 adipocytes. Journal of Integrative Medicine, 2017, 15(1): 56-63.
[5] Shi-li Jiang, Xu-dong Hu, Ping Liu. Immunomodulation and liver protection of Yinchenhao decoction against concanavalin A-induced chronic liver injury in mice. Journal of Integrative Medicine, 2015, 13(4): 262-268.
[6] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[7] Md. Asadujjaman, Ahmed Ullah Mishuk, Md. Aslam Hossain, Utpal Kumar Karmakar. Medicinal potential of Passiflora foetida L. plant extracts: Biological and pharmacological activities. Journal of Integrative Medicine, 2014, 12(2): 121-126.
[8] Marjan Nassiri-Asl, Taghi Naserpour Farivar, Esmail Abbasi, Hamid Reza Sadeghnia, Mehdi Sheikhi, Mina Lotfizadeh, Parisa Bazahang. Effects of rutin on oxidative stress in mice with kainic acid-induced seizure. Journal of Chinese Integrative Medicine, 2013, 11(5): 337-342.
[9] Md. Khirul Islam, Imran Mahmud, Sanjib Saha, Asit Baron Sarker, Himangsu Mondal, A.S.M. Monjur-Al-Hossain, Md. Anisuzzman. Preliminary pharmacological evaluation of Alocasia indica Schott tuber. Journal of Chinese Integrative Medicine, 2013, 11(5): 343-351.
[10] Sanjib Saha, Faroque Hossain, Md. Anisuzzman, Md. Khirul Islam. Pharmacological evaluation of Musa seminifera Lour. Fruit. Journal of Chinese Integrative Medicine, 2013, 11(4): 253-261.
[11] Sunil A. Nirmal, Jayashri M. Ingale, Shashikant R. Pattan, Sanjay B. Bhawar. Amaranthus roxburghianus root extract in combination with piperine as a potential treatment of ulcerative colitis in mice. Journal of Chinese Integrative Medicine, 2013, 11(3): 206-212.
[12] Mei-jiao Mao, Jun-ping Hu, Cong Wang, Yi-yi Zhang, Ping Liu. Effects of Chinese herbal medicine Guanxinkang on expression of PPARγ-LXRα-ABCA1 pathway in ApoE-knockout mice with atherosclerosis. Journal of Chinese Integrative Medicine, 2012, 10(7): 814-820.
[13] Lin Wang, Guan-liang Wang, Jia-han Liu, Di Li, De-zeng Zhu, Liang-neng Wu . Effects of ursolic acid in ameliorating insulin resistance in liver of KKAy mice via peroxisome proliferator-activated receptors α and γ. Journal of Chinese Integrative Medicine, 2012, 10(7): 793-799.
[14] Yi-tao Zhao , Xin-guang Zhang , Li Bai , Li-qing Li , Jian-er Yu . Effects of Chinese herbal medicine Pingchuan Formula on airway inflammation, interferon-γ and interleukin-4 in mice with asthma. Journal of Chinese Integrative Medicine, 2012, 10(7): 807-813.
[15] Dong-mei Zhang, Li-xia Lou, Ai-ming Wu, Xi-ying Lü, Zhao-juan Hu, Yan-hong Zhang, Hong-fang Liu. Effects of Astragalus membranaceus and Potentilla discolor mixture on insulin resistance and its related mRNA expressions in KKAy mice with type 2 diabetes. Journal of Chinese Integrative Medicine, 2012, 10(7): 821-826.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[2] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[5] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[6] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[7] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[8] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[9] Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[10] Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3