Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (1): 41-44.doi: 10.3736/jcim20080109

• Original Clinical Research • Previous Articles     Next Articles

Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis

Dong Yang1, Yong-ping Du1(), Qing Shen2, Wei Chen3, Yan Yu3, Guang-lei Chen3   

  1. 1. Department of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, Shannxi Province 710032, China
    2. Department of Nephrology, Paediatrics Hospital, Fudan University, Shanghai 210000, China
    3. Department of Nephrology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shannxi Province 710032, China
  • Received:2007-05-15 Online:2008-01-20 Published:2008-01-15
  • Contact: DU Yong-ping

Objective:To investigate the expression of α-smooth muscle action (α-SMA) in the renal tubulointerstitium in patients with kidney collateral stasis.

Methods:The expression of α-SMA in the renal biopsy specimens from 54 patients with kidney collateral stasis was examined by immunohistochemical method.

Results:The degree of kidney collateral stasis was increased with the increasing of the degree of renal interstitial fibrosis (P<0.05), and there was significant positive correlation between kidney collateral stasis and α-SMA expression (P<0.05).

Conclusion:Kidney collateral stasis is one of the main reasons of renal fibrosis. With the increasing of kidney collateral stasis, MFBs in the renal interstitium proliferate obviously, becoming one of the most important causes of renal interstitial fibrosis.

Key words: nephrosis, fibrosis, alpha actin, fibroblasts

CLC Number: 

  • R692

Table 1

Index of blood stasis, expression of α-SMA in the renal tubulointerstitium and the content of serum creatinine and blood urea nitrogen in different groups (?x±s)"

Group n Index of blood stasis Expression of α-SMA Level of serum creatinine (μmol/L) Level of blood urea nitrogen (mmol/L)
Light fibrosis 22 3.14±0.56 9.10±5.48 74.81±18.9 5.03±1.55
Moderate fibrosis 16 4.87±0.89* 15.00±6.69* 107.40±42.98 7.32±3.56
Severe fibrosis 16 6.63±0.86*△ 31.58±7.16*△ 267.54±178.19*△ 12.38±6.03*△

Figure 1

Pathology characteristics of renal biopsy specimens under microscope (Masson staining, ×200) A: Light fibrosis; B: Moderate fibrosis; C: Severe fibrosis."

Figure 2

Expression of α-SMA in the renal tubulointerstitium under microscope (Immunohistochemical staining, ×200) Light fibrosis; B: Moderate fibrosis; C: Severe fibrosis."

[1] Li C. Organ fibrosis: foundation and clinic. Beijing: People’s Medical Publishing House. 2003: 113-114. Chinese.
李才 . 器官纤维化: 基础与临床.北京:人民卫生出版社. 2003: 113-114.
[2] China Society of Integrated Traditional Chinese and Western Medicine. Diagnostic criteria for syndrome of blood stasis. Zhong Xi Yi Jie He Za Zhi. 1987; 7(3):129. Chinese.
中国中西医结合研究会. 血瘀证诊断标准.中西医结合杂志. 1987; 7(3):129.
[3] Ning YY, Wang JQ , Qu SL. Effect of emodin on human kidney fibroblast proliferation. Zhong Xi Yi Jie He Za Zhi. Qu SL. Effect of emodin on human kidney fibroblast proliferation. Zhong Xi Yi Jie He Za Zhi. 2000; 20(2):105-106. Chinese with abstract in English.
doi: 10.3321/j.issn:1003-5370.2000.02.009
宁英远, 王俭勤, 屈遂林 . 大黄素对人肾成纤维细胞增殖的影响.中西医结合杂志. 2000; 20(2):105-106.
doi: 10.3321/j.issn:1003-5370.2000.02.009
[4] Gao JY , Shi ZS. Study of Dahuang Zhechong Pill in treating the rats with chronic renal function failure. Zhong- guo Zhong Yi Yao Ke Ji. 1998; 5(2):73-74. Chinese.
髙峻钰, 时振声 . 大黄蜜虫丸治疗大鼠慢性肾功能衰竭的实验研究.中国中医药科技. 1998; 5(2):73-74.
[5] Hu HX, Liu ZM, Cai Q , et al. Effect of Yishen Huoxue Xiezhuo method on TGF-p in the nephridial tissue of the rats with renal function failure. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi. 2000; 6(6):369-371. Chinese.
胡海翔, 刘占民, 蔡庆 , 等. 益肾活血泻浊方对慢性肾衰大鼠肾组织TGF-p的影响.中国中医基础医学杂志. 2000; 6(6):369-371.
[1] Xue-qing Yu, Shu-guang Yang, Yang Xie, Jian-sheng Li. Traditional Chinese medicine in the treatment of idiopathic pulmonary fibrosis based on syndrome differentiation: Study protocol of exploratory trial. Journal of Integrative Medicine, 2020, 18(2): 163-168.
[2] Stefania Lamponi, Anna Maria Aloisi, Claudia Bonechi, Marco Consumi, Alessandro Donati, Gemma Leone, Claudio Rossi, Gabriella Tamasi, Luana Ghiandai, Ersilia Ferrini, Paolo Fiorenzani, Ilaria Ceccarelli, Agnese Magnani. Evaluation of in vitro cell and blood compatibility and in vivo analgesic activity of plant-derived dietary supplements. Journal of Integrative Medicine, 2019, 17(3): 213-220.
[3] Hoda Mojiri-Forushani, Ali Asghar Hemmati, Mohammad Amin Dehghani, Ali Reza Malayeri, Hossein Hassan Pour. Effects of herbal extracts and compounds and pharmacological agents on pulmonary fibrosis in animal models: A review. Journal of Integrative Medicine, 2017, 15(6): 433-441.
[4] JiaXia, Li-qunHe, XiaoSu. Interventional mechanisms of herbs or herbal extracts on renal interstitial fibrosis. Journal of Integrative Medicine, 2016, 14(3): 165-173.
[5] Takanobu Takata, Yoshiharu Motoo, Naohisa Tomosugi. Effect of Saikokeishito, a Kampo medicine, on hydrogen peroxide-induced premature senescence of normal human dermal fibroblasts. Journal of Integrative Medicine, 2014, 12(6): 495-503.
[6] Shu Dong, Shi-bing Su​. Advances in mesenchymal stem cells combined with traditional Chinese medicine therapy for liver fibrosis. Journal of Integrative Medicine, 2014, 12(3): 147-155.
[7] Qing-lan Wang, Yan-yan Tao , Li Shen , Hong-yan Cui , Cheng-hai Liu. Chinese herbal medicine Fuzheng Huayu recipe inhibits liver fibrosis by mediating the transforming growth factor-β1/Smads signaling pathway. Journal of Chinese Integrative Medicine, 2012, 10(5): 561-568.
[8] Zhou Xian-mei, Zhao Yang, He Cui-cui, Li Jian-xin. Preventive effects of Citrus reticulata essential oil on bleomycin-induced pulmonary fibrosis in rats and the mechanism. Journal of Chinese Integrative Medicine, 2012, 10(2): 200-209.
[9] Xiao-ning Wang, Qing Tao, Qin Feng, Jing-hua Peng, Ping Liu, Yi-yang Hu. Effects of Chinese herbal medicine Yiguanjian Decoction on collagen metabolism of hepatic tissues in rats with CCI4-induced liver fibrosis. Journal of Chinese Integrative Medicine, 2011, 9(6): 651-657.
[10] Wen Sun, Zhi Zhu, Jian Yu, Yong-hong Wang, Man Xiong, Xia Gao, Zhong-hua Zhao, Xue-guang Liu. Effects of Chinese herbal medicine Huaiqihuang Granule on nephrin and podocin expressions in renal tissues of rats with adriamycin-induced nephrosis. Journal of Chinese Integrative Medicine, 2011, 9(5): 546-552.
[11] Hua-qiang Zhai, Shuo-feng Zhang, Ming-chao Gao, Yang Liu, Min Ou, Fan-yun Meng, Yong-yan Wang. Effects of Herba Ephedra Sinicae and Fructus Schisandrae Chinensis on pathology of rats with bleomycin A5-induced idiopathic pulmonary fibrosis. Journal of Chinese Integrative Medicine, 2011, 9(5): 553-557.
[12] Yan Shen, Hong Wei, Chun-lan Jin. Effects of Chinese herbal medicine Xuefu Zhuyu Decoction on angiotensin Ⅱ-induced proliferation and extracellular matrix synthesis of rat cardiac fibroblasts. Journal of Chinese Integrative Medicine, 2011, 9(3): 313-319.
[13] Yan-yan Tao, Qing-lan Wang, Ji-li Yuan, Li Shen, Cheng-hai Liu. Effects of vitamin E on mercuric chloride-induced renal interstitial fibrosis in rats and the antioxidative mechanism. Journal of Chinese Integrative Medicine, 2011, 9(2): 201-208.
[14] Ling-ying Liu, Xiao-dong Chen, Bo-yu Wu, Qiong Jiang. Influence of Aloe polysaccharide on proliferation and hyaluronic acid and hydroxyproline secretion of human fibroblasts in vitro. Journal of Chinese Integrative Medicine, 2010, 8(3): 256-262.
[15] Yong-mei Liu, Rui-hua Liu, Wen-jun Liu, Li Liu , Zhi-kui Wu, Yu-ying Chen. Effects of Chinese herbal medicine Yiqi Huoxue Formula on TGF-p/smad signal transduction pathway and connective tissue growth factor in rats with renal interstitial fibrosis. Journal of Chinese Integrative Medicine, 2010, 8(12): 1165-1173.
Full text



[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[4] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[5] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[6] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[7] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[8] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[9] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[10] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8