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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (12): 1159-1163.doi: 10.3736/jcim20091213

• Original Experimental Research • Previous Articles     Next Articles

Effects of Yanggan Lidan Granule on insulin resistance in guinea pigs with induced cholesterol gallstones

Bang-jiang Fanga,Shuang Zhoub,Xin-jun Peia,Jin-yang Huanga,Bao-jin Chena,Yun Genga,Li-kun Yangb   

  1. a Department of Emergency, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    b School of Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China
  • Received:2009-06-29 Accepted:2009-08-14 Online:2009-12-20 Published:2009-12-15
  • Contact: Shuang Zhou E-mail:zhoushuang8008@163.com

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Objective

To observe the effects of Yanggan Lidan Granule (YGLDG), a compound traditional Chinese herbal medicine, on insulin resistance in guinea pigs with induced cholesterol gallstones.
Methods

Eighty guinea pigs were randomly divided into normal control group, untreated group, YGLDG group and ursodeoxycholic acid (UDCA) group, with 20 guinea pigs in each group. Except the normal control group, gallstones were induced by high-cholesterol diet in the guinea pigs. The guinea pigs in the normal control group and the untreated group were administered with normal saline. UDCA and YGLDG were given to the guinea pigs in the corresponding groups for seven weeks. Eight guinea pigs of each group were used to measure the glucose infusion rate (GIR) by using hyperinsulinemic-euglycemic clamp technique. At the end the guinea pigs were killed and their gallstone formation was observed.
Results

The gallstones in guinea pigs were identified as cholesterol stones by qualitative analysis through infrared spectrum. The incidence rate of cholelithiasis of the untreated group was 82.35%. The GIR of guinea pigs in the untreated group was obviously lowered down as compared with the normal control group. Compared with the untreated group, the GIRs of the YGLDG group and the UDCA group were obviously increased, especially in the YGLDG group. Conclusion: YGLDG may improve insulin resistance in guinea pigs with cholesterol gallstones by elevating GIR obviously.

Key words: Compound (TCD), Yanggan Lidan Granule, Gallstone, Glucose clamp technique, Guinea pigs

Table 1

Incidence of cholelithiasis in four groups"

Group n Number of rats with gallstone Incidence rate of cholelithiasis (%)
Normal control 19 1 5.26
Untreated 17 14 82.35
UDCA 18 7 38.89
YGLD 18 5 27.78

Table 2

Blood glucose in four groups tested by hyperinsulinemic-euglycemic clamp technique ($\bar{x}$±s, mmo1/L)"

Group n Blood glucose at different time points
–60 min 0 min 80 min 100 min 110 min 120 min
Normal control 19 5.1±0.8 5.3±0.4 5.0±0.2 5.2±0.1 5.2±0.2 5.4±0.2
Untreated 17 6.9±1.2** 5.3±0.2 5.2±0.2 5.3±0.2 5.5±0.2 5.5±0.2
UDCA 18 6.5±0.7 5.3±0.1 5.0±0.2 5.2±0.1 5.2±0.2 5.4±0.2
YGLD 18 6.1±0.5 5.3±0.2 5.2±0.2 5.3±0.2 5.5±0.2 5.5±0.2

Table 3

GIR in four groups tested by hyperinsulinemic-euglycemic clamp technique [$\bar{x}$±s, mg/(kg·min)]"

Group n GIR at different time points
0 min 80 min 100 min 110 min
Normal control 19 32.1±1.7 34.5±1.6 34.4±1.6 34.7±1.7
Untreated 17 12.7±1.3 12.2±1.6** 12.9±1.8** 12.0±1.9**
UDCA 18 20.5±2.9 23.1±2.6△△ 23.1±2.2△△ 23.2±1.7△△
YGLD 18 27.7±2.1 28.3±1.9△△▲▲ 28.6±1.3△△▲▲ 28.9±1.1△△▲▲
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