养肝利胆颗粒对胆囊胆固醇结石豚鼠胰岛素抵抗的干预作用

doi:10.3736/jcim20091213

Abstract

Objective

To observe the effects of Yanggan Lidan Granule (YGLDG), a compound traditional Chinese herbal medicine, on insulin resistance in guinea pigs with induced cholesterol gallstones.
Methods

Eighty guinea pigs were randomly divided into normal control group, untreated group, YGLDG group and ursodeoxycholic acid (UDCA) group, with 20 guinea pigs in each group. Except the normal control group, gallstones were induced by high-cholesterol diet in the guinea pigs. The guinea pigs in the normal control group and the untreated group were administered with normal saline. UDCA and YGLDG were given to the guinea pigs in the corresponding groups for seven weeks. Eight guinea pigs of each group were used to measure the glucose infusion rate (GIR) by using hyperinsulinemic-euglycemic clamp technique. At the end the guinea pigs were killed and their gallstone formation was observed.
Results

The gallstones in guinea pigs were identified as cholesterol stones by qualitative analysis through infrared spectrum. The incidence rate of cholelithiasis of the untreated group was 82.35%. The GIR of guinea pigs in the untreated group was obviously lowered down as compared with the normal control group. Compared with the untreated group, the GIRs of the YGLDG group and the UDCA group were obviously increased, especially in the YGLDG group. Conclusion: YGLDG may improve insulin resistance in guinea pigs with cholesterol gallstones by elevating GIR obviously.

Key words: Compound (TCD), Yanggan Lidan Granule, Gallstone, Glucose clamp technique, Guinea pigs

Bang-jiang Fang, Shuang Zhou, Xin-jun Pei, Jin-yang Huang, Bao-jin Chen, Yun Geng , Li-kun Yang. Effects of Yanggan Lidan Granule on insulin resistance in guinea pigs with induced cholesterol gallstones. Journal of Chinese Integrative Medicine, 2009, 7(12): 1159-1163.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20091213

http://www.jcimjournal.com/EN/Y2009/V7/I12/1159

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References 17

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Group	n	Blood glucose at different time points
Group	n	–60 min	0 min	80 min	100 min	110 min	120 min
Normal control	19	5.1±0.8	5.3±0.4	5.0±0.2	5.2±0.1	5.2±0.2	5.4±0.2
Untreated	17	6.9±1.2^**	5.3±0.2	5.2±0.2	5.3±0.2	5.5±0.2	5.5±0.2
UDCA	18	6.5±0.7	5.3±0.1	5.0±0.2	5.2±0.1	5.2±0.2	5.4±0.2
YGLD	18	6.1±0.5	5.3±0.2	5.2±0.2	5.3±0.2	5.5±0.2	5.5±0.2

Group	n	GIR at different time points
Group	n	0 min	80 min	100 min	110 min
Normal control	19	32.1±1.7	34.5±1.6	34.4±1.6	34.7±1.7
Untreated	17	12.7±1.3	12.2±1.6^**	12.9±1.8^**	12.0±1.9^**
UDCA	18	20.5±2.9	23.1±2.6^△△	23.1±2.2^△△	23.2±1.7^△△
YGLD	18	27.7±2.1	28.3±1.9^△△▲▲	28.6±1.3^△△▲▲	28.9±1.1^△△▲▲

Effects of Yanggan Lidan Granule on insulin resistance in guinea pigs with induced cholesterol gallstones

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