SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (4): 294-298.doi: 10.3736/jcim20050414

• Original Experimental Research • Previous Articles     Next Articles

Protective effects of Chailing Guiqi Decoction combined with lumbrukinase on renal function in rats with adriamycin nephropathy

Fen-fang Zhang1,Xi-dong Wang2,Huan-fang Fan1,Zhi-qiang Chen1,Zhi-wei Yin1,Wei Zhang3   

  1. 1. Institute of Integrated Traditional Chinese and Western Medicine, Hebei Medical University, Shijiazhuang, Heibei Province 050017, China
    2. Department of Laboratory Medicine, Second Hospital, Hebei Medical University, Shijiazhuang, Heibei Province 050005, China
    3. Second Clinic, Affiliated Agency of Hebei Provice, Shijiazhuang, Heibei Province 050051, China
  • Received:2004-09-20 Online:2005-07-20 Published:2005-07-20
  • Contact: Zhi-qiang Chen E-mail:chenzhqiang@tom.com

RichHTML

2

PDF (PC)

112

Cited

78

Like

Objective

To study the protective efffects of Chailing Guiqi Decoction (CLGQD) combined with lumbrukinase on renal function in rats with adriamycin nephropathy.

Methods

Thirty-six SD rats were randomly divided into four groups: normal control group, untreated group, simvastatin-treated group and CLGQD -treated group. Adriamycin nephropathy was induced by intravenous injection with 5 mg/kg adriamycin. After seven-day treatment, quantitative measurement of 24-h urine protein was determined with trichloroacetic acid, and serum total protein (TP), albumin (Alb), total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), creatinine (Cr) and blood urea nitrogen (BUN) were assessed using automatic biochemistry analyzer. The pathomorphological changes of renal tissues were observed with light and electron microscopes.

Results

In the untreated group, the 24-h urine protein excretion, serum TC, TG, LDL, Cr and BUN were significantly higher than those in the normal control group (P<0.05 or P<0.01), while the serum TP, Alb, HDL were significantly lower than those in the normal control group (P<0.01). In the CLGQD-treated group, the 24-h urine protein excretion, serum TC, TG, LDL, Cr and BUN were significantly lower as compared with those in the untreated group (P<0.05 or P<0.01), while the serum TP, Alb and HDL were significantly higher as compared with those in the untreated group (P<0.05 or P<0.01). The pathomorphological findings of the renal tissues under the light microscope in the untreated group showed focal segmental glomerulosclerosis in a few of glomerulus, degenerated and swelled proximal tubular epithelial cells, proteins in cast formation in some renal tubules and scattered fibrosis in interstitial tissues of the kidney, while the electron microscope images showed the fusion of foot processes in glomerular epithelial cells. The pathomorphological changes in the CLGQD-treated group were slighter than those in the untreated group.

Conclusion

CLGQD combined with lumbrukinase can reduce proteinuria, regulate lipid metabolism, protect renal function, and delay progressive renal damage in rats.

Key words: Chailing Guiqi Decoction, Adriamycin nephropathy, Proteinuria, Lipid metabolism, Hyperlipidemia, Renal tissue, Pathomorphology

CLC Number: 

  • R287.3

Tab 1

24-h urine protein excretion at the 2nd,3rd,4th,5th,6th and 7th weekends （$\bar{x}$±s, mg/24 h）"

Group n 24- urine protein excretion at different weekend
2nd weekend 3nd weekend 4nd weekend 5nd weekend 6nd weekend 7nd weekend
Normal control 6 10.03±2.59 10.85±2.08 22.61±4.98 17.75±3.11 21.70±6.41 16.56±3.95
Untreated 10 13.62±4.41 136.16±72.03** 240.70±124.13** 280.6±172.06** 273.53±153.82** 262.03±188.12**
Simvastatin-treated 10 11.65±2.31 78.89±50.24** 93.86±38.05**△△ 102.53±72.68*△△ 106.47±55.2**△△ 108.57±56.24**△▲
CLGQD-treated 10 11.76±2.43 84.19±52.18** 98.78±37.75**△△ 119.89±53.68**△ 116.51±54.32*△△▲ 109.71±57.71**△▲

Tab 2

Test results of serum TP、Alb、BUN and Cr （$\bar{x}$±s）"

Group n TP (g/L) Alb (g/L) BUN (mmol/L) Cr (μmol/L)
Normal control 6 68.54±5.36 37.58±4.32 6.47±1.34 52.55±6.22
Untreated 9 61.40±5.57* 29.14±3.30** 12.82±2.86** 61.76±8.03*
Simvastatin-treated 9 66.41±4.24△ 32.06±1.47**△ 9.46±2.11**△ 52.57±8.43△
CLGQD-treated 9 68.10±2.65△△ 32.12±0.92**△ 9.34±2.17*△ 53.70±4.96△

Tab 3

Test results of serum TG、TC、HDL and LDL （$\bar{x}$±s, mmol/L）"

Group n TG TC HDL LDL
Normal control 6 1.27±0.32 1.84±0.46 1.55±0.68 0.32±0.14
Untreated 9 1.80±0.21** 5.47±2.62** 0.76±0.21** 1.37±0.73**
Simvastatin-treated 9 1.46±0.36△ 3.54±1.56*△ 1.27±0.44△△ 0.75±0.23**△
CLGQD-treated 9 1.45±0.19△△ 3.03±1.23*△ 1.20±0.32△△ 0.79±0.24**△
[1] 谭永淑, 王峥 . 阿霉素诱发大鼠肾病的病理观察[J]. 临床与实验病理学杂志, 1992,8(3):224-225
[2] 从玉文, 刘耀明, 陈家佩 . 蚓激酶的研究进展[J]. 中国生化药物杂志, 2000,21(3):159-161
doi: 10.3969/j.issn.1005-1678.2000.03.027
[3] 张国平, 钱睿哲, 杨诗春 , 等. 蚓激酶抑制血小板集聚的观察[J]. 中华医学杂志, 1998,78(5):394-395
[4] 董光富 . 蛋白尿和慢性肾功能衰竭进展关系研究近况[J]. 国外医学·泌尿系统分册, 1998,18(4):165-168
[5] 牛建英 . 蛋白尿损害肾脏的机制及其干预措施[J]. 肾脏病与透析肾移植杂志, 2003,12(1):66-69
[6] Burton C, Harris KP . The role of proteinuria in the progression of chronic renal failure[J]. Am J Kidney Dis, 1996,27(6):765-775
doi: 10.1016/S0272-6386(96)90512-0 pmid: 8651239
[7] Praga M, Morales E . Renal damage associated with proteinuria[J]. Kidney Int Suppl, 2002,( 82):42-46
doi: 10.1046/j.1523-1755.62.s82.9.x pmid: 12410854
[8] Wheeler DC, Bernard DB . Lipid abnormalities in the nephrotic syndrome: causes, consequences and treatment[J]. Am J Kidney Dis, 1994,23(3):331-346
doi: 10.1016/S0272-6386(12)80994-2
[9] 张桂贤, 常敏毅 . 柴胡成分抗肾炎效果的研究[J]. 国医论坛, 1992,7(2):45-47
[10] 邵淑丽 . 柴胡、姜黄对小白鼠实验性高脂血症的预防作用[J]. 中医药学报, 2002,30(4):59
doi: 10.3969/j.issn.1002-2392.2002.04.061
[11] 梁云, 崔若兰 . 柴胡皂苷-d治疗抗肾小球基膜型肾炎的实验研究[J]. 第二军医大学学报, 1999,20(7):416-419
doi: 10.3321/j.issn:0258-879X.1999.07.003
[12] 徐安平, 崔若兰 . 柴胡在肾小球疾病中治疗作用的研究进展[J]. 国外医药·植物药分册, 1996,11(2):55-58
[13] 汪德清, 沈文梅 . 黄芪有效成分对氧自由基清除作用的ESR研究[J]. 生物化学与生物物理进展, 1996,23(3):260-262
[14] 房喻, 胡道道, 李晓军 . 黄芩甙及其铜(Ⅱ)、锌(Ⅱ)配合物对超氧自由基的清除作用[J]. 生物化学杂志, 1991,7(6):753-756
[15] 谢玲, 杨凌红, 李晓惠 . 当归药理作用研究进展[J]. 中医药研究, 2000,16(6):56-58
[16] 吴畏, 阳崇德, 刘才平 . 地龙药理作用的研究新进展[J]. 中药药理与临床, 2002,18(2):48-49
doi: 10.3969/j.issn.1001-859X.2002.02.029
[17] 黎莉, 甘明, 黎祥胜 , 等. 地龙类药材药理作用比较[J]. 中药材, 1997,20(7):361-363
[18] 冯文茹, 孙向军, 胡人杰 . 中药茯苓的药理研究及临床应用[J]. 天津医科大学学报, 1995,1(2):95-97
[19] Yamashita T, Kawashima S , Miwa Y, et al.A 3-hydroxy-3methylglutaryl co-enzyme A reductase inhibitor reduces hypertensive nephrosclerosis in stroke-prone spontaneously hypertensive rats[J]. J Hypertens, 2002,20(12):2465-2473
doi: 10.1097/00004872-200212000-00027
[20] Elisaf M, Mikhailidis DP . Statins and renal function[J]. Angiology, 2002,53(5):493-502
doi: 10.1177/000331970205300501
[21] Oda H, Keane WF . Recent advances in statins and the kidney[J]. Kidney Int Suppl, 1999,( 71):S2-S5
doi: 10.1046/j.1523-1755.1999.07101.x pmid: 10412725
[22] Yoshinura A, Nemoto T, Sugenoya Y , et al. Effect of simvastatin on proliferative nephritis and cell-cycle protein expression[J]. Kidney Int Suppl, 1999,( 71):S84-S87
doi: 10.1046/j.1523-1755.1999.07121.x pmid: 10412745
[1] Zeinab Vafaeipour, Bibi Marjan Razavi, Hossein Hosseinzadeh. Effects of turmeric (Curcuma longa) and its constituent (curcumin) on the metabolic syndrome: An updated review. Journal of Integrative Medicine, 2022, 20(3): 193-203.
[2] Meng-ya Shan, Ying Dai, Xiao-dan Ren, Jing Zheng, Ke-bin Zhang, Bin Chen, Jun Yan, Zi-hui Xu. Berberine mitigates nonalcoholic hepatic steatosis by downregulating SIRT1-FoxO1-SREBP2 pathway for cholesterol synthesis. Journal of Integrative Medicine, 2021, 19(6): 545-554.
[3] Ya-lei Liu, Zhen-yu Zhou, Min Gao, Guang Ji, Cheng Huang, Sheng-jie Fan. Therapeutic effects of herbal formula Huangqisan on metabolic disorders via SREBF1, SCD1 and AMPK signaling pathway. Journal of Integrative Medicine, 2021, 19(2): 167-176.
[4] Omar Farid, Farid Khallouki, Morad Akdad, Andrea Breuer, Robert Wyn Owen, Mohamed Eddouks. Phytochemical characterization of polyphenolic compounds with HPLC–DAD–ESI–MS and evaluation of lipid-lowering capacity of aqueous extracts from Saharan plant Anabasis aretioides (Coss & Moq.) in normal and streptozotocin-induced diabetic rats. Journal of Integrative Medicine, 2018, 16(3): 185-191.
[5] Cheng Huang​. Natural modulators of liver X receptors. Journal of Integrative Medicine, 2014, 12(2): 76-85.
[6] Jin Jin, Yang Zhang, Wen-xiang Hu, Zheng-yao Zhang, Nan-nan Xu, Qiu-li Zhou. Effects of Huanglian Jiedu Decoction on blood lipid metabolism and its related gene expressions in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2010, 8(3): 275-279.
[7] Yan-yi Ren, Han-lin Gong, Wen-Fu Tang, Mei-hua Wan, Jian-lei Zhao, Xi Huang. Dachengqi Decoction induces pancreatic acinar cell apoptosis in experimental acute pancreatitis in rats. Journal of Chinese Integrative Medicine, 2009, 7(7): 651-.
[8] Ping Xie, Li-hui Deng, Zhao-da Zang, Qing Xia, Zong-wen Huang, Xiao-nan Yang, Jun-ming Jiang. Clinical study of Yihuo Qingxia method in treating hyperlipoidemia-related severe acute pancreatitis in early stage. Journal of Chinese Integrative Medicine, 2008, 6(3): 262-265.
[9] Jian-nan Song, Jun-lian Liu, Xiang-zhong Fang, Yuan-hui Hu, Yan Lei, Xiao-hong Niu, Gang Wu, Bao-sheng Chen, Ya-luan Ma, Bing Chen, Hong jin. Relationship between plasma protein expression profiles and states of Zang-Fu organs in patients with phlegm or blood stagnation syndromes due to hyperlipidemia and atherosclerosis. Journal of Chinese Integrative Medicine, 2008, 6(12): 1233-1237.
[10] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92.
[11] Li-qun He, Jun Li, Qi Li. High-protein diet and high-grade proteinuria aggravate the progression of chronic renal failure in rats. Journal of Chinese Integrative Medicine, 2007, 5(3): 333-337.
[12] Qiang Fu, Li-qun He, He-xin Cao. Effects of Jianpi Qinghua Recipe on oxygen radicals and transforming growth factor β1 in renal tissue in a rat model of chronic renal failure with hyperlipidemia. Journal of Chinese Integrative Medicine, 2006, 4(4): 408-412.
[13] Qin Feng, Yi-yang Hu. Syndromes and pathological mechanism of insulin resistance in traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2005, 3(1): 63-65.
[14] Xue-mei Liu, Fu-huo Wu. Comparison of animal models of hyperlipidemia. Journal of Chinese Integrative Medicine, 2004, 2(2): 132-134.
[15] Xu-min Hou, Huan-chun Ni, Xin-ping Luo, Hai-ming Shi, Wei-hu Fan. Effect of Zhenju Jiangya Tablet on the endothelial function of hyperlipidemic rabbits. Journal of Chinese Integrative Medicine, 2004, 2(2): 111-114.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3