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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (4): 294-298.doi: 10.3736/jcim20050414

• Original Experimental Research • Previous Articles     Next Articles

Protective effects of Chailing Guiqi Decoction combined with lumbrukinase on renal function in rats with adriamycin nephropathy

Fen-fang Zhang1,Xi-dong Wang2,Huan-fang Fan1,Zhi-qiang Chen1,Zhi-wei Yin1,Wei Zhang3   

  1. 1. Institute of Integrated Traditional Chinese and Western Medicine, Hebei Medical University, Shijiazhuang, Heibei Province 050017, China
    2. Department of Laboratory Medicine, Second Hospital, Hebei Medical University, Shijiazhuang, Heibei Province 050005, China
    3. Second Clinic, Affiliated Agency of Hebei Provice, Shijiazhuang, Heibei Province 050051, China
  • Received:2004-09-20 Online:2005-07-20 Published:2005-07-20
  • Contact: Zhi-qiang Chen E-mail:chenzhqiang@tom.com

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Objective

To study the protective efffects of Chailing Guiqi Decoction (CLGQD) combined with lumbrukinase on renal function in rats with adriamycin nephropathy.

Methods

Thirty-six SD rats were randomly divided into four groups: normal control group, untreated group, simvastatin-treated group and CLGQD -treated group. Adriamycin nephropathy was induced by intravenous injection with 5 mg/kg adriamycin. After seven-day treatment, quantitative measurement of 24-h urine protein was determined with trichloroacetic acid, and serum total protein (TP), albumin (Alb), total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), creatinine (Cr) and blood urea nitrogen (BUN) were assessed using automatic biochemistry analyzer. The pathomorphological changes of renal tissues were observed with light and electron microscopes.

Results

In the untreated group, the 24-h urine protein excretion, serum TC, TG, LDL, Cr and BUN were significantly higher than those in the normal control group (P<0.05 or P<0.01), while the serum TP, Alb, HDL were significantly lower than those in the normal control group (P<0.01). In the CLGQD-treated group, the 24-h urine protein excretion, serum TC, TG, LDL, Cr and BUN were significantly lower as compared with those in the untreated group (P<0.05 or P<0.01), while the serum TP, Alb and HDL were significantly higher as compared with those in the untreated group (P<0.05 or P<0.01). The pathomorphological findings of the renal tissues under the light microscope in the untreated group showed focal segmental glomerulosclerosis in a few of glomerulus, degenerated and swelled proximal tubular epithelial cells, proteins in cast formation in some renal tubules and scattered fibrosis in interstitial tissues of the kidney, while the electron microscope images showed the fusion of foot processes in glomerular epithelial cells. The pathomorphological changes in the CLGQD-treated group were slighter than those in the untreated group.

Conclusion

CLGQD combined with lumbrukinase can reduce proteinuria, regulate lipid metabolism, protect renal function, and delay progressive renal damage in rats.

Key words: Chailing Guiqi Decoction, Adriamycin nephropathy, Proteinuria, Lipid metabolism, Hyperlipidemia, Renal tissue, Pathomorphology

CLC Number: 

  • R287.3

Tab 1

24-h urine protein excretion at the 2nd,3rd,4th,5th,6th and 7th weekends （$\bar{x}$±s, mg/24 h）"

Group n 24- urine protein excretion at different weekend
2nd weekend 3nd weekend 4nd weekend 5nd weekend 6nd weekend 7nd weekend
Normal control 6 10.03±2.59 10.85±2.08 22.61±4.98 17.75±3.11 21.70±6.41 16.56±3.95
Untreated 10 13.62±4.41 136.16±72.03** 240.70±124.13** 280.6±172.06** 273.53±153.82** 262.03±188.12**
Simvastatin-treated 10 11.65±2.31 78.89±50.24** 93.86±38.05**△△ 102.53±72.68*△△ 106.47±55.2**△△ 108.57±56.24**△▲
CLGQD-treated 10 11.76±2.43 84.19±52.18** 98.78±37.75**△△ 119.89±53.68**△ 116.51±54.32*△△▲ 109.71±57.71**△▲

Tab 2

Test results of serum TP、Alb、BUN and Cr （$\bar{x}$±s）"

Group n TP (g/L) Alb (g/L) BUN (mmol/L) Cr (μmol/L)
Normal control 6 68.54±5.36 37.58±4.32 6.47±1.34 52.55±6.22
Untreated 9 61.40±5.57* 29.14±3.30** 12.82±2.86** 61.76±8.03*
Simvastatin-treated 9 66.41±4.24△ 32.06±1.47**△ 9.46±2.11**△ 52.57±8.43△
CLGQD-treated 9 68.10±2.65△△ 32.12±0.92**△ 9.34±2.17*△ 53.70±4.96△

Tab 3

Test results of serum TG、TC、HDL and LDL （$\bar{x}$±s, mmol/L）"

Group n TG TC HDL LDL
Normal control 6 1.27±0.32 1.84±0.46 1.55±0.68 0.32±0.14
Untreated 9 1.80±0.21** 5.47±2.62** 0.76±0.21** 1.37±0.73**
Simvastatin-treated 9 1.46±0.36△ 3.54±1.56*△ 1.27±0.44△△ 0.75±0.23**△
CLGQD-treated 9 1.45±0.19△△ 3.03±1.23*△ 1.20±0.32△△ 0.79±0.24**△
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