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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (4): 408-412.doi: 10.3736/jcim20060418

• Original Experimental Research • Previous Articles     Next Articles

Effects of Jianpi Qinghua Recipe on oxygen radicals and transforming growth factor β1 in renal tissue in a rat model of chronic renal failure with hyperlipidemia

Qiang Fu, Li-qun He, He-xin Cao   

  1. Department of Nephrology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China
  • Online:2006-07-31 Published:2006-07-15

Objective

To evaluate the effects of Jianpi Qinghua Recipe (JPQHR) on oxygen radicals and transforming growth factor β1 (TGFβ1) in renal tissue in a rat model of chronic renal failure with hyperlipidemia.

Methods

Chronic renal failure with hyperlipidemia was induced in rats in untreated group and JPQHR-treated group by 5/6 nephrectomy and high fat diet. Then the rats in these two groups were fed distilled water and JPQHR respectively for eight weeks. The rats in normal control group received no specific interventions. After eight weeks of treatment, the levels of 24 h urine protein (Upr), blood urea nitrogen (BUN) and serum creatinine (Cr), cholesterol (Ch), triglycerides (TG), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) of rats in these three groups were examined. The activity of superoxide dismutase (SOD), contents of malondialdehyde (MDA) and non-esterified fatty acids (NEFA) and expression level of TGFβ1 mRNA in renal tissue of rats in each groups were also determined.

Results

The levels of 24 Upr, BUN and serum Cr, Ch, TG and LDL in the JPQHR-treated group were significantly lower than those in the untreated group. The contents of MDA and NEFA and the expression level of TGFβ1 mRNA in the JPQHR-treated group were also significantly lower than those in the untreated group, while the activity of SOD was significantly increased in the JPQHR-treated group as compared with that in the untreated group.

Conclusion

The results indicate that JPQHR can improve the renal function of rats with chronic renal failure and hyperlipidemia by regulating lipid metabolism, maintaining balance between prooxidants and antioxidants and reducing expression of TGFβ1 mRNA in renal tissue.

Key words: Jianpi Qinghua Recipe, Chronic kidney failure, Hyperlipidemia, Oxygen radicals, Transforming growth factor beta

CLC Number: 

  • R692.5

Table 1

Levels of 24 h Upr, serum Cr and BUN of rats in different groups(ヌ±S )"

Group n BUN (mmol/L) sCr (μmol/L) 24 h Upr (mg/24 h)
Normal control 8 9.26±0.37 35.38±5.07 23.26±4.44
Untreated 7 32.32±10.80** 132.83±62.37** 63.17±14.66**
JPQHR-treated 6 18.59±7.19△△ 72.88±26.63△△ 38.77±9.46△△

Table 2

Blood levels of Ch, TG, HDL and LDL of rats in different groups(ヌ±S , mmol/L)"

Group n Ch TG HDL LDL
Normal control 8 1.56±0.25 0.40±0.07 0.96±0.19 0.35±0.07
Untreated 7 3.56±1.81** 1.65±1.48** 2.03±0.72** 0.86±0.50**
JPQHR-treated 6 2.55±0.40△△ 0.89±0.17△△ 1.64±0.24 0.54±0.09△△

Table 3

Activity of SOD and contents of MDA and NEFA in renal tissue of rats in different groups(ヌ±S )"

Group n SOD (μU/L) MDA (pmol/L) NEFA (mmol/L)
Normal control 8 409.94±50.28 12.90±3.11 19.95±15.64
Untreated 7 68.08±46.60** 27.66±1.84** 162.33±47.39**
JPQHR-treated 6 216.43±181.25△△ 21.68±3.40△△ 38.65±17.10△△

Table 4

Expression level of TGFβ1 mRNA in renal tissue of rats in different groups(ヌ±S )"

Group n TGFβ1 mRNA
Normal control 8 0.24±0.03
Untreated 7 1.04±0.41**
JPQHR-treated 6 0.36±0.22△△
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