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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (6): 642-646.doi: 10.3736/jcim20070608

• Original Experimental Research • Previous Articles     Next Articles

Modulation of Jianjining Recipe on differential protein expression in rats with experimental autoimmune myasthenia gravis

Ping Liu1, Xu-feng Ding1, Yi-yi Zhang1, Jian Qiao2   

  1. 1. Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China
    2. Neurology Institute,Huashan Hospital,Fudan University,Shanghai 200040,China
  • Online:2007-11-20 Published:2007-11-15

Objective: To study the modulation of Jianjining Recipe (JJNR), a traditional Chinese compound herbal medicine for invigorating spleen and kidney on differential protein expression in spleen of rats with experimental autoimmune myasthenia gravis (EAMG).Methods: EAMG rats were randomly divided into four groups: untreated group, JJNR-treated group, Qiangji Jianli capsule (QJJLC, a traditional Chinese compound herbal medicine)-treated group and prednisolone acetate (PA)-treated group. After therapeutic intervention with the above drugs for four consecutive weeks, the level of differential protein expression was analyzed by two-dimensional electrophoresis and matrix assisted laser desorption/ionization time-of-flight mass spectrometry.Results: Twelve differential proteins were identified by comparing EAMG rsts and normal rats. The levels of allograft inflammatory factor-1, peroxiredoxin Ⅰ and actin-related protein 2/3 complex subunit 5 were significantly regulated by JJNR (P<0.01). These proteins were closely associated with immune response and cell movement.Conclusion: The results suggest that there are differential protein expressions between EAMG rats and normal rats. Furthermore, as a Chinese medicine prescription with effect of invigorating spleen and kidney, JJNR can effectively regulate the levels of some EAMG-related protein expression.

Key words: myasthenia gravis, experimental autoimmune, spleen, electrophoresis, gel, two-dimensional

CLC Number: 

  • R746.1

Table 1

Relevant data searched from NCBI database"

No MS identificaition Mw/kD PI Protein score
8129 Allograft inflammatory factor-1 (AIF-1) 16.817 7.88 85
7227 Peroxiredoxin Ⅰ (PRDX Ⅰ) 22.095 8.27 170
1410 40S ribosomal protein SA (RSSA 40) 33.001 4.8 147
2601 Actin, cytoplasmic type 8 (ACT 8) 41.821 5.31 146
1302 Phenylanyl-tRNA synthetase alpha chain (SYF A) 38.712 5.8 54
2202 COP9 signalosome complex subunit 8 (CSN 8) 23.241 5.09 92
7228 Cytochrome c-551 (C 551) 25.252 9.53 70
8132 Aldehyde dehydrogenase, mitochondrial (ALDH 2) 54.299 5.83 143
5625 Aldehyde dehydrogenase, mitochondrial (ALDH 2) 54.299 5.83 208
1210 Signal transducer and activator of transcription 1 (STAT 1) 88.111 5.9 84
2718 60 kDa heat shock protein, mitochondrial precursor (HSP 60) 60.917 5.91 206
4102 Actin-related protein 2/3 complex subunit 5 (ARPC 5) 16.179 5.47 150

Figure 1

Two-dimensional electrophoresis map of rats in different groups A: Normal group; B: Untreated group; C: Jianjining-treated group; D: Qiangji Jianli Capsule-treated group; E: Prednisolone acetate-treated group."

Table 2

Relative intensity of disease-related proteins expressed in different groups (?x±s)"

Group No. 8129 (AIF-1) No. 7227 (PRDX Ⅰ) No. 2601 (ACT 8) No. 4102 (ARPC 5)
Normal 1 115.8±182.9 1 847.2±274.2 1 611.8±433.5 4 063.6±208.7
Untreated 2 412.4±156.2** 3 980.9±510.9** 598.0±370.5* 1 958.5±72.7**
Jianjining Recipe-treated 1 180.6±220.0△△ 1 838.4±141.3△△ 334.5±129.4 4 296.6±103.9△△
Qiangji Jianli Capsule-treated 1 166.3±124.3△△ 1 600.9±584.9△△ 1 427.1±517.1△△ 1 359.9±1 095.5
Prednisolone acetate-treated 2 028.0±380.1 3 455.1±659.8 2 000.5±621.4△△ 2 470.3±473.6
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