脑络欣通对局灶性脑缺血再灌注大鼠热休克蛋白及碱性成纤维细胞生长因子表达的影响

doi:10.3736/jcim20040411

Abstract

Objective: To investigate the possible beneficial effects of administration of Naoluo Xintong Recipe (NLXT) on the expression of HSP70 and bFGF in rats subjected to ischemia-reperfusion with qi deficiency and blood stasis syndrome.Methods: We blindly randomized 40 male Wistar rats into 5 groups which were categorized as follows: (1) sham-operated group; (2) rats subjected to transient middle cerebral artery (MCA) oclusion with qi deficiency and blood stasis syndrome (control group); (3) NLXT-treated group; (4) NLXT-pretreated group; (5) Nao'an Capsule-treated group. Focal brain ischemia was induced by the intraluminal suture MCA occlusion method after qi deficiency and blood stasis syndrome had been made. The rats' brains were harvested at the 24 h of reperfusion after 2 h of MCA occlusion. The expression of HSP70 and bFGF was measuredby immunohistochemical staining.Results: Strong HSP70 immunoreactivity in neurons was observed in NLXT-treated and NLXT-pretreated groups as compared to control group,and increased bFGF immunoreactivity in neurons was observed in the ischemic hemisphere in NLXT-pretreated group in comparison with the ischemic hemisphere in the control group (P<0.01). NLXT has stronger effects on HSP70 and bFGF than Nao'an Capsule.Conclusion: The neuroprotective effects of Naoluo Xintong Recipe may be related to its up-regulation of HSP70 and bFGF in the rat brain after focal cerebral ischemia.

Key words: Naoluo Xintong Recipe, brain ischemia, qi deficiency and blood stasis syndrome, HSP70, bFGF

CLC Number:

R285.5

Jian Wang, Xin Liu. Effects of Naoluo Xintong Recipe on expression of HSP70 and bFGF in focal ischemia-reperfusion rats. Journal of Chinese Integrative Medicine, 2004, 2(4): 271-273.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20040411

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Group	n	Quantified HSP70-positive cells
Sham-operated	7	2.71±2.81
Control	5	210.44±58.75**
NLXT-treated	6	327.00±51.43**^ΔΔ▲
NLXT-pretreated	8	338.56±43.79**^ΔΔ▲
Nao'an Capsule-treated	7	269.00±75.18**

Group	n	Quantified bFGF-positive cells
Sham-operated	7	66.50±23.73
Control	5	116.83±17.62*
NLXT-treated	6	140.17±64.76**
NLXT-pretreated	8	204.33±36.27**^ΔΔ▲▲
Nao'an Capsule-treated	7	117.50±30.85*

Effects of Naoluo Xintong Recipe on expression of HSP70 and bFGF in focal ischemia-reperfusion rats

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