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Journal of Integrative Medicine ›› 2016, Vol. 14 ›› Issue (6): 465-472.doi: 10.1016/S2095-4964(16)60280-7

• Research Article • Previous Articles     Next Articles

Investigation of the cytotoxicity, antioxidative and immune-modulatory effects of Ligusticum porteri (Osha) root extract on human peripheral blood lymphocytes

Khanh Nguyen, Jean Sparks, Felix O. Omoruyi   

  1. Department of Life Sciences, College of Science and Engineering, Texas A&M University-Corpus Christi, Texas 78412, USA
  • Received:2016-05-13 Accepted:2016-08-26 Online:2016-11-15 Published:2016-11-15
  • Contact: Felix O. Omoruyi; E-mail: felix.omoruyi@tamucc.edu

Objective

Ligusticum porteri is a traditional Native American herb. The roots of L. porteri are traditionally used in the treatment of many diseases, however, its cytotoxicity, antioxidative and immune-modulatory effects need to be investigated. In this study, we evaluated the effects of the root extract at different doses on human peripheral blood lymphocytes (PBLs).

Methods

The lymphocytes were incubated with different concentrations of the root extracts (0, 50, 100, 200, and 400 μg/mL) and harvested every 6 h for 2 d (P<0.05). The protective effect of the herb against oxidative damage was determined by inducing oxidative stress with the administration of 50 μmol/L of hydrogen peroxide (H2O2).

Results

Treatments with L. porteri at 200 and 400 μg/mL increased the viability of PBLs. The deleterious effect of H2O2 was ameliorated by 400 μg/mL L. porteri treatment. Addition of 400 μg/mL L. porteri reduced lipid peroxidation in stressed PBLs by 94% (P<0.05). Treatment with 400 μg/mL of L. porteri resulted in a 26.4% increase of reduced glutathione levels. Activities of superoxide dismutase and catalase increased by 17.5% and 55.2% respectively, when stressed PBLs were treated with 400 μg/mL L. porteri for 2 d (P<0.05). Treatment with 400 μg/mL L. porteri increased interferon-γ and interleukin-2 expressions in H2O2-challenged PBLs (P<0.05), however, the root extract did not cause a significant difference in interleukin-10 levels compared to the control (P>0.05).

Conclusion

The findings suggest that L. porteri might be a potential immune-modulating agent involving protective effects against oxidative damage.

Key words: Ligusticum porteri, Root extract, Cytotoxicity, Immunologic, Oxidative stress, Immunemodulatory, Human peripheral blood lymphocytes

1 Kindscher K, Yang J, Long Q, Craft R, Loring H. Harvest sustainability study of wild populations of Osha, Ligusticum porteri. (2013) [ 2016-05-01]. 2013) [ 2016-05-01]. http://nativeplants.ku.edu/wpcontent/ uploads/2013/12/Osha-Report-2013.pdf.
2 Wilson M. Medicinal plant fact sheet: Ligusticum porteri/ Osha. (2007) [2016-05-01]. 2007) [2016-05-01]. http://www.pollinator.org/ Resources/Osha%20-%20Ligusticum.draft.pdf.
3 León A, Toscano RA, Tortoriello J, Delgado G.Phthalides and other constituents from Ligusticum porteri; sedative and spasmolytic activities of some natural products and derivatives. Nat Prod Res.2011; 25(13): 1234-1242.
4 United States Department of Agriculture. Ligusticum porteri JM Coult. & Rose. (2014) [2016-05-01]. 2014) [2016-05-01]. http://plants.usda. gov/core/profi le?symbol=LIPO.
5 Shu MH, Appleton D, Zandi K, AbuBakar S. Antiinfl ammatory, gastroprotective and anti-ulcerogenic effects of red algae Gracilaria changii(Gracilariales, Rhodophyta) extract.BMC Complement Altern Med. 2013; 13: 61.
6 Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB.Oxidative stress, inflammation, and cancer: how are they linked?Free Radic Biol Med. 2010; 49(11): 1603-1616.
7 Beltran O. Investigation of the anti-mycobacterial and cytotoxic effect of three medicinal plants used in the traditional treatment of tuberculosis in northern Mexico and the Southwest United States. (2008) [2016-05-01]. 2008) [2016-05-01]. http:// search.proquest.com/docview/304372126.
8 Chkhikvishvili I, Sanikidze T, Gogia N, Mchedlishvili T, Enukidze M, Machavariani M, Vinokur Y, Rodov V.Rosmarinic acid-rich extracts of summer savory (Satureja hortensis L.) protect Jurkat T cells against oxidative stress. Oxid Med Cell Longev. 2013; 2013: 456253.
9 Wang L, Lin SQ, He YL, Liu G, Wang ZY.Protective effects of quercetin on cadmium-induced cytotoxicity in primary cultures of rat proximal tubular cells.Biomed Environ Sci. 2013; 26(4): 258-267.
10 Genet S, Kale RK, Baquer NZ.Alterations in antioxidant enzymes and oxidative damage in experimental diabetic rat tissues: effect of vanadate and fenugreek (Trigonellafoenum graecum). Mol Cell Biochem. 2002; 236(1-2): 7-12.
11 Ellman GL.Tissue sulfhydryl groups. Arch Biochem Biophys.1959; 82(1): 70-77.
12 Gautam N, Das S, Kar Mahapatra S, Chakraborty SP, Kundu PK, Roy S.Age associated oxidative damage in lymphocytes.Oxid Med Cell Longev. 2010; 3(4): 275-282.
13 Nawaz SK, Hasnain S.Effects of noise exposure on catalase activity of growing lymphocytes. Bosn J Basic Med Sci.2011; 11(4): 219-222.
14 Saito Y, Nishio K, Ogawa Y, Kimata J, Kinumi T, Yoshida Y, Noguchi N, Niki E.Turning point in apoptosis/necrosis induced by hydrogen peroxide. Free Radic Res.2006; 40(6): 619-630.
15 Reth M.Hydrogen peroxide as second messenger in lymphocyte activation. Nat Immunol.2002; 3(12): 1129-1134.
16 Keong CK, Nadarajah VD, Lee TJ.Development of a purification method of pure primary lymphocytes for cell viability assays. Malays J Med Sci.2007; 14(1): 38-45.
17 El-Aal H. Lipid peroxidation end-products as a key of oxidative stress: effect of antioxidant on their production and transfer of free radicals. (2008). [2016-05-01]. 2008). [2016-05-01]. http:// www.intechopen.com/books/lipid-peroxidation/lipidperoxidation- end-products-as-a-key-of-oxidative-stresseffect- of-antioxidant-on-their-producti.
18 Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J.Free radicals and antioxidants in normal physiological functions and human disease.Int J Biochem Cell Biol. 2007; 39(1): 44-84.
19 Staal FJ, Ela SW, Roederer N, Anderson MT, Herzenberg LA.Glutathione defi ciency and human immunodefi ciency virus infection.Lancet. 1992; 339(8798): 909-912.
20 Robinson MK, Rodrick ML, Jacobs DO, Rounds JD, Collins KH, Saporoschetz IB, Mannick JA, Wilmore DW.Glutathione depletion in rats impairs T-cell and macrophage immune function. Arch Surg.1993; 128(1): 29-34.
21 Aukrust P, Svardal AM, Müller F, Lunden B, Berge RK, Frøland SS.Decreased levels of total and reduced glutathione in CD4+ lymphocytes in common variable immunodeficiency are associated with activation of the tumor necrosis factor system: possible immunopathogenic role of oxidative stress.Blood. 1995; 86(4): 1383-1391.
22 Dröge W, Schulze-Osthoff K, Mihm S, Galter D, Schenk H, Eck HP, Roth S, Gmünder H.Functions of glutathione and glutathione disulfi de in immunology and immunopathology.FASEB J. 1994; 8(14): 1131-1138.
23 Limón-Pacheco J, Gonsebatt ME.The role of antioxidants and antioxidant-related enzymes in protective responses to environmentally induced oxidative stress. Mutat Res.2009; 674(1-2): 137-147.
24 Ng PC, Li K, Wong RP, Chui K, Wong E, Li G, Fok TF.Proinfl ammatory and anti-infl ammatory cytokine responses in preterm infants with systemic infections. Arch Dis Child Fetal Neonatal Ed.2003; 88(3): F209-F213.
25 Blachère NE, Morris HK, Braun D, Saklani H, Di Santo JP, Darnell RB, Albert ML.IL-2 is required for the activation of memory CD8+ T cellsvia antigen cross-presentation. J Immunol. 2006; 176(12): 7288-7300.
26 Souza KL, Gurgul-Convey E, Elsner M, Lenzen S.Interaction between pro-inflammatory and antiinflammatory cytokines in insulin-producing cells.J Endocrinol. 2008; 197(1): 139-150.
27 Schroder K, Hertzog PJ, Ravasi T, Hume DA.Interferongamma: an overview of signals, mechanisms and functions. J Leukoc Biol.2004; 75(2): 163-189.
28 Zhou X, Schmidtke P, Zepp F, Meyer CU.Boosting interleukin-10 production: therapeutic effects and mechanisms.Curr Drug Targets Immune Endocr Metabol Disord. 2005; 5(4): 465-475.
29 Ostrowski K, Rohde T, Asp S, Schjerling P, Pedersen BK.Pro- and anti-inflammatory cytokine balance in strenuous exercise in humans.J Physiol. 1999; 515(Pt1): 287-291.
30 Del- ángel M, Nieto A, Ramírez-Apan T, Delgado G.Anti-inflammatory effect of natural and semi-synthetic phthalides. Eur J Pharmacol.2015; 752: 40-48.
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