中药地椒乙醇提取物对人白血病细胞增殖的抑制作用

doi:10.3736/jcim20050513

Abstract

Objective

To screen the anti-tumor fraction of ethanol extracts from Thymus quinquecostatus Celak and investigate its anti-tumor effect on human leukemia cell line.

Methods

Ethyl acetate, n-butanol and acetone fractions were separated from the ethanol extracts of wild Thymus quinquecostatus Celak. Growth inhibiting effects of these extracts on human leukemia cell lines K562 and HL-60 were determined by live cell counting and cell growth curve analysis. The possible anti-tumor mechanism was studied by morphological analysis with norcantharidin as a positive control.

Results

Ethyl acetate fraction could significantly inhibit the proliferations of K562 and HL-60 cells, and the inhibiting effect depended on the concentration of ethyl acetate fraction. Ethyl acetate fraction could induce apoptosis of K562 and HL-60 cells. The n-butanol and acetone fractions had no significant inhibiting effect on K562 and HL-60 cells.

Conclusion

Ethyl acetate fraction is the major anti-tumor fraction in ethanol extracts from Thymus quinquecostatus Celak.

Key words: Thymus quinquecostatus Celak, Ethanol extract, Ethyl acetate fraction, Antineoplastic agents, K562, HL-60

CLC Number:

R286.91

Zhen-xiao Sun, Ying-hui Zhang, Shuang Cheng, Qing-wen Ma, Shan-li Guo, Jin-bao Zhang. Anti-tumor effect of ethanol extracts from Thymus quinquecostatus Celak on human leukemia cell line. Journal of Chinese Integrative Medicine, 2005, 3(5): 382-385.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20050513

http://www.jcimjournal.com/EN/Y2005/V3/I5/382

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Figures/Tables 7

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References 11

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Group	n	K562 cells	HL-60 cells
Blank control	4	69.3±2.4	160.8±18.7
1% DMSO control	2	55.3±7.6	142.5±10.5
Acetone fraction (20 μg/ml)	2	50.0±4.6	202.0±9.0
n-butanol fraction (200 μg/ml)	2	51.0±13.0	131.0±9.0
ethyl acetate fraction (100 μg/ml)	2	25.0±3.1*	88.0±18.0*

Anti-tumor effect of ethanol extracts from Thymus quinquecostatus Celak on human leukemia cell line

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