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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (5): 361-363.doi: 10.3736/jcim20040515

• Original Experimental Research • Previous Articles     Next Articles

Effects of purslane herb on stress ability of aging mice induced by D-galactose

Chen Ling   

  1. Department of Biology,School of Life Science, Fudan University, Shanghai 200433, China
  • Received:2004-01-18 Online:2004-09-20 Published:2018-10-20

Objective: To investigate the effects of purslane herb aquenous extracts (PHAS) on the stress ability of aging mice induced by D-galactose.Methods: We odserved the survival time to hypoxia and heat survival rate of the mice treated with different doses of PHAS and vitamin E. The contents of lipofuscin and malondialdehyde (MDA), and the activity of superoxide dismutase (SOD) and catalase (CAT) in the brain and liver of the mice were tested.Results: As compared with vitamin E, three doses of PHAS (1.6, 0.8 and 0.4 ml/d) prolonged the survival time to hypoxia and the pole climbing time and increased the heat survival rate, and the 0.8 ml/d PHAS had the best effect.In the group of 0.8 ml/d PHAS, the activity of SOD and CAT decreased less, and the contents of lipofuscin and MDA decreased significantly.The effect of vitamin E was not as good as the PHAS.Conclusion: PHAS can prevent the stress ability of the aging mice.One of its mechanisms may be increasing the activity of SOD and CAT, hence decreasing the damage of the oxidation products to the body.

Key words: purslane herb, aging, stress, antioxidation

CLC Number: 

  • R283.1

Tab 1

Stress ability of aging mice induced by D-galactose"

Group Pole climbing time
(n=30,$\bar{x}$±s, min)
Survival time to hypoxia
(n=10,$\bar{x}$±s, min)
Heat survival rate
(n=10, %)
Normal mice 20.5±4.3 18.2±2.8 100
Aging mice 12.0±2.5* * 16.0±1.7* 10
Vitamin E 15.8±3.3* 15.2±2.0* 20
High dose purslane herb 21.0±5.1 ▲▲ 19.2±1.8▲▲ 80
Middle dose purslane herb 22.7±5.3 ▲▲ 21.5±2.2▲▲ 80
Low dose purslanc herb 18.8±4.0▲▲ 18.7±2.3 50

Tab 2

Activity of SOD and CAT in aging mice induced by D-galactose ($\bar{x}$±s )"

Group n SOD (nU/mg pro) CAT
Cerebral cortex Liver Blood (U/g Hb) Liver (U/mg pro)
Normal mice 10 82.3±16.8 102.2±45.8 83.7±9.3 272.1±43.2
Aging mice 10 62.5±19.6* * 60.1±26.9* 69.5±10.6* * 239.6±48.0
Vitamin E 10 71.5±16.3 78.8±25.4 71.2±10.5* * 245.7±34.6
High dose purslane herb 10 78.6±9.2 89.6±27.7 83.0±4.3▲▲ 305.8±34.6▲▲
Middle dose purslane herb 10 81.6±18.9 92.6±22.8 87.9±5.4▲▲ 265.7±30.6
Low dose purslane herb 10 75.0±14.7 82.4±21.3 85.7±5.1▲▲ 241.7±27.3

Tab 3

Contents of MDA and lipofusin in aging mice induced by D-galactose ($\bar{x}$±s )"

Group n Lipofusin (fluorescent reading number) MDA (nmol/mg pro)
Cerebral cortex Liver Cerebral cortex Liver
Normal mice 10 65.7±3.6 4.2±0.7 3.2±0.8 1.1±0.3
Aging mice 10 75.2±6.3 5.2±0.3 5.6±1.1 1.5±0.4
Vitamin E 10 69.6±6.9 4.7±0.3 4.6±0.3 0.9±0.3
High dose purslane herb 10 66.9±7.7 4.8±0.5 3.2±0.8 1.2±0.3
Middle dose purslane herb 10 69.5±7.1 4.5±0.6 4.0±0.6 1.1±0.2
Low dose purslane herb 10 74.0±4.8 4.7±0.8 4.4±1.0 1.3±0.3
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