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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (9): 956-959.doi: 10.3736/jcim20080916

• Original Experimental Research • Previous Articles     Next Articles

Effects of tetrandrine on nuclear factor-kappaB expression in leukemia multidrug-resistant cell line K562/A02

Bao-an Chen1(), Ai-ling Su1, Jian Cheng1, Hui-hui Zhao1, Guo-hong Li1, Xue-mei Wang2   

  1. 1. Department of Hematology, Zhongda Hospital, Southeast University, Nanjing, Jiangsu Province 210009, China
    2. State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering, Southeast University, Nanjing, Jiangsu Province 210096, China
  • Received:2008-01-24 Online:2008-09-20 Published:2008-09-15

Objective: To investigate the effects of tetrandrine (Tet) on nuclear factor kappaB (NF-κB) expression in leukemia cell line K562 and multidrug-resistant K562/A02 cell line.
Methods: The activations of NF-κB in K562 and K562/A02 cells and the effects of 1 μmol/L Tet on NF-κB expression were determined by immunocytochemistry and Western blot assay.
Results: Tet had no effect on NF-κB expression in K562 cells after 6- and 12-hour treatment (P>0.05), and K562/A02 cells displayed higher level of NF-κB protein expression than their parental K562 cells (P<0.01). Tet could significantly down-regulate NF-κB protein expression and nuclear translocation in K562/A02 cells shown by immunocytochemistry and Western blot, and this decrease became more significant after 12-hour treatment than after at 6-hour treatment (P<0.05).
Conclusion: Activation of NF-κB may have relaton to multidrug resistance of K562/A02 cell line. And the inhibition of NF-κB activation by Tet leads to multidrug resistance reversal in K562/A02 cell line.

Key words: leukemia, multidrug resistance, tetrandrine, nuclear factor kappaB

CLC Number: 

  • R966

Figure 1

Expressions of NF-κB protein of K562/A02 cells cultured in medium containing Tet for 6 hours and 12 hours 1: K562/A02 cell group; 2: K562 cell group; 3: K562/A02 plus 6-hour Tet group; 4: K562/A02 cell group; 5: K562 group; 6: K562/A02 plus 12-hour Tet group."

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